The mechanism of delaying starch digestion by luteolin

木犀草素 化学 氢键 淀粉 猝灭(荧光) 荧光光谱法 傅里叶变换红外光谱 圆二色性 范德瓦尔斯力 疏水效应 荧光 结晶学 生物化学 分子 有机化学 类黄酮 化学工程 工程类 物理 量子力学 抗氧化剂
作者
Yiling Zhao,Ming Wang,Jinsheng Zhang,Chunhong Xiong,Ganhui Huang
出处
期刊:Food & Function [Royal Society of Chemistry]
卷期号:12 (23): 11862-11871 被引量:13
标识
DOI:10.1039/d1fo02173g
摘要

In this study, the mechanisms of the delay of starch digestion by luteolin were revealed by studying the luteolin-PPA (porcine pancreatic α-amylase) interaction and luteolin-starch interaction. The luteolin-PPA interaction was investigated by inhibitory kinetics analysis, fluorescence quenching, circular dichroism (CD), Fourier transform infrared (FT-IR) spectroscopy and molecular docking. The results of the inhibitory kinetics revealed that luteolin was a mixed-type inhibitor of PPA and that the inhibitory action was reversible. Fluorescence spectroscopy (including fluorescence quenching and thermodynamics) and molecular docking analyses indicated that hydrogen bonds and hydrophobic forces were the main forces between PPA and luteolin. CD and FT-IR spectroscopy analyses showed that the interaction between luteolin and PPA changed the secondary structure of PPA and induced a decline in its activity. In addition, the luteolin-starch interaction was also studied using UV-visible absorption and X-ray diffraction analyses. These indicated that luteolin could bind with PPA, and that hydrogen bonds and van der Waals forces may be present. Overall, luteolin delayed starch digestion not only by binding with PPA but also by binding with starch. Thus, luteolin has the potential to prevent and control diabetes by being added into starch-based food to delay starch digestion.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
hyx9504发布了新的文献求助10
1秒前
2秒前
2秒前
2秒前
2秒前
2秒前
3秒前
Ray-Q完成签到,获得积分10
4秒前
4秒前
春夏秋冬完成签到 ,获得积分10
5秒前
miaolingcool发布了新的文献求助30
5秒前
YYONE发布了新的文献求助10
6秒前
MgZn发布了新的文献求助10
6秒前
6秒前
开心发布了新的文献求助10
7秒前
7秒前
毛毛发布了新的文献求助30
7秒前
8秒前
8秒前
调调完成签到,获得积分10
8秒前
Orange应助科研通管家采纳,获得100
8秒前
yar应助科研通管家采纳,获得10
8秒前
今后应助科研通管家采纳,获得10
8秒前
8秒前
8秒前
JamesPei应助科研通管家采纳,获得10
8秒前
8秒前
pluto应助王若琪采纳,获得10
8秒前
传奇3应助可耐的青雪采纳,获得10
9秒前
10秒前
10秒前
忧郁平蝶关注了科研通微信公众号
10秒前
云清发布了新的文献求助20
10秒前
10秒前
CodeCraft应助大胆的如容采纳,获得30
11秒前
刘可完成签到,获得积分20
11秒前
shine发布了新的文献求助10
11秒前
十一发布了新的文献求助10
12秒前
巧克力餐包完成签到,获得积分10
12秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975165
求助须知:如何正确求助?哪些是违规求助? 3519595
关于积分的说明 11198781
捐赠科研通 3255912
什么是DOI,文献DOI怎么找? 1798001
邀请新用户注册赠送积分活动 877343
科研通“疑难数据库(出版商)”最低求助积分说明 806298