The mechanism of delaying starch digestion by luteolin

木犀草素 化学 氢键 淀粉 猝灭(荧光) 荧光光谱法 傅里叶变换红外光谱 圆二色性 范德瓦尔斯力 疏水效应 荧光 结晶学 生物化学 分子 有机化学 类黄酮 化学工程 工程类 物理 量子力学 抗氧化剂
作者
Yiling Zhao,Ming Wang,Jinsheng Zhang,Chunhong Xiong,Ganhui Huang
出处
期刊:Food & Function [Royal Society of Chemistry]
卷期号:12 (23): 11862-11871 被引量:13
标识
DOI:10.1039/d1fo02173g
摘要

In this study, the mechanisms of the delay of starch digestion by luteolin were revealed by studying the luteolin-PPA (porcine pancreatic α-amylase) interaction and luteolin-starch interaction. The luteolin-PPA interaction was investigated by inhibitory kinetics analysis, fluorescence quenching, circular dichroism (CD), Fourier transform infrared (FT-IR) spectroscopy and molecular docking. The results of the inhibitory kinetics revealed that luteolin was a mixed-type inhibitor of PPA and that the inhibitory action was reversible. Fluorescence spectroscopy (including fluorescence quenching and thermodynamics) and molecular docking analyses indicated that hydrogen bonds and hydrophobic forces were the main forces between PPA and luteolin. CD and FT-IR spectroscopy analyses showed that the interaction between luteolin and PPA changed the secondary structure of PPA and induced a decline in its activity. In addition, the luteolin-starch interaction was also studied using UV-visible absorption and X-ray diffraction analyses. These indicated that luteolin could bind with PPA, and that hydrogen bonds and van der Waals forces may be present. Overall, luteolin delayed starch digestion not only by binding with PPA but also by binding with starch. Thus, luteolin has the potential to prevent and control diabetes by being added into starch-based food to delay starch digestion.
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