自愈水凝胶
基质凝胶
细胞外基质
组织工程
材料科学
细胞生物学
层粘连蛋白
神经生长因子
生长因子
神经组织工程
神经干细胞
干细胞
生物
细胞
生物化学
遗传学
受体
高分子化学
作者
Oana Dobre,Mariana Azevedo González Oliva,Giuseppe Ciccone,Sara Trujillo,Aleixandre Rodrigo‐Navarro,D. Venters,Virginia Llopis‐Hernandez,Massimo Vassalli,Cristina González‐García,Matthew J. Dalby,Manuel Salmerón‐Sánchez
标识
DOI:10.1002/adfm.202010225
摘要
Abstract Laminins (LMs) are important structural proteins of the extracellular matrix (ECM). The abundance of every LM isoform is tissue‐dependent, suggesting that LM has tissue‐specific roles. LM binds growth factors (GFs), which are powerful cytokines widely used in tissue engineering due to their ability to control stem cell differentiation. Currently, the most commonly used ECM mimetic material in vitro is Matrigel, a matrix of undefined composition containing LM and various GFs, but subjected to batch variability and lacking control of physicochemical properties. Inspired by Matrigel, a new and completely defined hydrogel platform based on hybrid LM‐poly(ethylene glycol) (PEG) hydrogels with controllable stiffness (1–25 kPa) and degradability is proposed. Different LM isoforms are used to bind and efficiently display GFs (here, bone morphogenetic protein (BMP‐2) and beta‐nerve growth factor (β‐NGF)), enabling their solid‐phase presentation at ultralow doses to specifically target a range of tissues. The potential of this platform to trigger stem cell differentiation toward osteogenic lineages and stimulate neural cells growth in 3D, is demonstrated. These hydrogels enable 3D, synthetic, defined composition, and reproducible cell culture microenvironments reflecting the complexity of the native ECM, where GFs in combination with LM isoforms yield the full diversity of cellular processes.
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