化学
拟杆菌
发酵
多糖
肠道菌群
唾液
单糖
碳水化合物
糖醛酸
食品科学
维管菌
消化(炼金术)
半乳糖
细菌
生物
微生物学
体外
生物化学
色谱法
链球菌
遗传学
作者
Yidan Wang,Guijie Chen,Yujia Peng,Rui Ying,Xiaoxiong Zeng,Ye Hong
标识
DOI:10.1016/j.lwt.2018.10.028
摘要
Crude polysaccharides from Coralline pilulifera (CPPS) were purified by DEAE Sepharose Fast Flow to afford four purified fractions of CPPS-1, CPPS-2, CPPS-3 and CPPS-4. The physicochemical property and digestion under simulated saliva, gastric, small intestinal conditions and fermentation of CPPS-3 were investigated in vitro. CPPS-3 was composed of glucose and galactose, its molecular weight was 716 kDa, the contents of carbohydrate, protein, uronic acid and sulfuric radical were 67.84%, 1.00%, 0.38% and 9.07%, respectively. There was no significant difference on the molecular weight and content of reducing sugars, and no monosaccharides were released after saliva, gastric and small intestine digestion, indicating that CPPS-3 could not be digested under the simulated digestion conditions. After fermentation for 24 h in vitro, the carbohydrate content was decreased to 30.76%, suggesting that CPPS-3 could be broken down and utilized by gut microbiota. Some health-promoting gut microbiota such as Bacteroides, Megamonas and Veillonella were significantly enhanced, suggesting that CPPS-3 could regulate the composition of gut microbiota. What's more, CPPS-3 could promote the productions of short-chain fatty acids (22.17 and 16.17 mmol/L at 24 h for CPPS-3 and control, respectively), which could contribute to host health. Therefore, CPPS-3 is expected to be developed as functional foods.
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