适体
检出限
化学
线性范围
牛血清白蛋白
组合化学
吸附
动态光散射
胶体金
色谱法
纳米颗粒
纳米技术
材料科学
分子生物学
生物
有机化学
作者
Jinseong Jeon,Hunho Jo,Jin Her,Hyungjun Youn,Jonghoon Park,Jihoon Jo,Jiseon Lee,Chulhun L. Chang,Changill Ban
出处
期刊:ChemBioChem
[Wiley]
日期:2019-07-04
卷期号:20 (17): 2236-2240
被引量:11
标识
DOI:10.1002/cbic.201900109
摘要
The soluble interleukin-2 receptor α (sIL-2Rα) is a broad indicator of clinical disease activity in various inflammatory diseases. Here we have developed, for the first time, a rapid, washing-free colorimetric aptasensor based on a sIL-2Rα aptamer (Kd =1.33 nm). The aptasensor was fabricated with Au nanoparticles (AuNPs) adsorbing sIL-2Rα aptamers. On addition of sIL-2Rα, the aptamers become desorbed from the AuNPs, and this in turn weakens the absorption corresponding to AuNP-catalyzed oxidation of ortho-phenylenediamine (oPD) with H2 O2 . The aptasensor was characterized by TEM imaging, ζ potential measurements, dynamic light scattering (DLS) analysis, and UV/Vis spectrometry, followed by further optimization. The fabricated sensor exhibited great analytical performance, with a linear range of 1 to 100 nm and a detection limit of 1 nm both in buffer and in spiked human serum within 25 min. Other proteins, such as bovine serum albumin (BSA), IL-17Rα, IL-5Rα, IL-13Rα2 , and CD166, showed negligible effects on the aptasensor. Thanks to the great advantages of the aptamers and AuNPs, this aptasensor provides a rapid, simple, and inexpensive process that might offer insights into various diagnostic applications of sIL-2Rα.
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