Objective To investigate the species composition, diversity and prevalence of the infection of Gasterophilus botfly larvae in horses from Duolun county, Inner Mongolia Autonomous Region.Methods Adult horses were treatedorally using ivermectin, the botfly larvae were collected from the feces and identified.Results Six species in the genus Gasterophilus were collected from each individual horse and with the following prevalence: G. intestinalis 53.04%,G. nasalis 24.00%, G. haemorrhoidalis 10.61%, G. pecorum 6.96%, G. nigricornis 3.13%, G. inermis 2.26%.Conclusion The species of Gasterophilus in Duolun county are abundant, and the horse gastric myiasis is prevalent in this area.