转染
病毒学
生物
人类白细胞抗原
病毒
异源的
分子生物学
抗原
K562细胞
链霉菌
T细胞
T细胞受体
细胞
细胞培养
免疫学
基因
免疫系统
遗传学
作者
Lloyd D’Orsogna,Ellen M. W. van der Meer‐Prins,Yvonne M. Zoet,Dave L. Roelen,Ilias Doxiadis,Frans H.J. Claas
标识
DOI:10.1007/978-1-61779-842-9_19
摘要
The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target “detector” cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of “heterologous immunity” from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.
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