树枝状大分子
化学
荧光
支化(高分子化学)
DNA
生物物理学
动力学
荧光相关光谱
指数增长
纳米技术
高分子化学
生物化学
分子
有机化学
光学
材料科学
数学分析
物理
生物
量子力学
数学
作者
Xiaoming Feng,I‐Ming Hsing
摘要
We present a nonlinear hybridization chain reaction (HCR) system in which a trigger DNA initiates self-sustained assembly of quenched double-stranded substrates into fluorescent dendritic nanostructures. During the process, an increasing number of originally sequestered trigger sequences labeled with fluorescent reporters are freed up from quenched substrates, leading to chain-branching growth of the assembled DNA dendrimers and an exponential increase in the fluorescence intensity. The triggered assembly behavior was examined by PAGE analysis, and the morphologies of the grown dendrimers were verified by AFM imaging. The exponential kinetics of the fluorescence accumulation was also confirmed by time-dependent fluorescence spectroscopy. This method adopts a simple sequence design strategy, the concept of which could be adapted to program assembly systems with higher-order growth kinetics.
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