生物
RNA依赖性RNA聚合酶
T7 RNA聚合酶
核糖核酸
RNA聚合酶Ⅰ
聚合酶
抄写(语言学)
RNA编辑
小核RNA
RNA聚合酶
RNA聚合酶Ⅱ
分子生物学
转录泡
转录因子ⅡD
作者
Nadège Arnaud-Barbe,Valérie Cheynet-Sauvion,Guy Oriol,Bernard Mandrand,François Mallet
标识
DOI:10.1093/nar/26.15.3550
摘要
Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA-and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was able to use templates containing RNA-RNA duplexes under standard transcription conditions. Implications of remnants of such RNA-dependent activities for T7 DNA-dependent polymerase are discussed.
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