Research progress in promoters of Pichia pastoris

Pichia pastoris expression system is one of the most successful expression systems for foreign proteins currently. The development and utilization of its promoters have also drawn wide attention. Alcohol oxidase 1 promoter is the most widely used promoter in Pichia pastoris. Many other promoters, such as glyceraldehyde-3-phosphate dehydrogenase promoter, formaldehyde dehydrogenase 1 promoter, have also been exploited and used in the need of foreign protein expression. The origin and characteristics of promoters, as well as foreign protein expression of Pichia pastoris are summarized in this paper to provide references for the choice of promoters. Key words: Pichia; Promoter; Transcription, genetic