Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

脂类学 失调 生物 肠道菌群 厚壁菌 微生物群 代谢组学 代谢组 拟杆菌 脂质代谢 微生物学 免疫学 生物信息学 细菌 生物化学 遗传学 16S核糖体RNA
作者
Yiyi Li,Hongmei Yan,Yao-Wei Zhang,Ping Fang,Lu Yu,Qianyu Li,Cuiting Liu,Yuwen Xie,Keli Chen,Feng Ye,Kai Wang,Longhua Chen,Yi Ding
出处
期刊:Frontiers in Cellular and Infection Microbiology [Frontiers Media]
卷期号:10 被引量:61
标识
DOI:10.3389/fcimb.2020.541178
摘要

Radiation enteritis (RE) is a common complication in cancer patients receiving radiotherapy. Although studies have shown the changes of this disease at clinical, pathological and other levels, the dynamic characteristics of local microbiome and metabolomics are hitherto unknown. We aimed to examine the multi-omics features of the gut microecosystem, determining the functional correlation between microbiome and lipid metabolites during RE activity. By delivering single high-dose irradiation, a RE mouse model was established. High-throughput16S rDNA sequencing and global lipidomics analysis were performed to examine microbial and lipidomic profile changes in the gut microecosystem. Spearman correlation analysis was used to determine the functional correlation between bacteria and metabolites. Clinical samples were collected to validate the above observations. During RE activity, the intestinal inflammation of the mice was confirmed by typical signs, symptoms, imaging findings and pathological evidences. 16S datasets revealed that localised irradiation dramatically altered the gut microbial composition, resulting in a decrease ratio of Bacteroidetes to Firmicutes. Lipidomics analysis indicated the remarkable lipidomic profile changes in enteric epithelial barrier, determining that glycerophospholipids metabolism was correlated to RE progression with the highest relevance. Spearman correlation analysis identified that 5 bacteria-metabolite pairs showed the most significant functional correlation in RE, including Alistipes-PC(36:0e), Bacteroides-DG(18:0/20:4), Dubosiella-PC(35:2), Eggerthellaceae-PC(35:6), Escherichia-Shigella-TG(18:2/18:2/20:4). These observations were partly confirmed in human specimens. Our study provided a comprehensive description of microbiota dysbiosis and lipid metabolic disorders in RE, suggesting strategies to change local microecosystem to relieve radiation injury and maintain homeostasis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
星辰大海应助do0采纳,获得10
2秒前
tt完成签到 ,获得积分10
3秒前
浅辰完成签到,获得积分10
4秒前
大气萤完成签到,获得积分20
5秒前
5秒前
我ppp完成签到 ,获得积分10
5秒前
6秒前
易燃物品完成签到,获得积分10
6秒前
Hello应助Ther采纳,获得10
8秒前
CherylZhao完成签到,获得积分10
9秒前
Galato发布了新的文献求助10
10秒前
颜愫完成签到,获得积分10
10秒前
安详向日葵完成签到 ,获得积分10
11秒前
拼搏的白云完成签到,获得积分10
11秒前
852应助hhh采纳,获得10
11秒前
李白白白完成签到,获得积分10
11秒前
王手完成签到,获得积分10
11秒前
12秒前
一人完成签到,获得积分10
13秒前
do0完成签到,获得积分10
14秒前
yar应助xlz110采纳,获得10
14秒前
NexusExplorer应助落寞凌波采纳,获得10
16秒前
量子星尘发布了新的文献求助10
19秒前
123完成签到 ,获得积分10
19秒前
哈哈呵完成签到,获得积分10
19秒前
19秒前
Rylee完成签到,获得积分10
19秒前
Jiro完成签到,获得积分10
21秒前
我ppp发布了新的文献求助60
22秒前
23秒前
纳米酶催化完成签到,获得积分10
24秒前
24秒前
John完成签到,获得积分10
24秒前
李小强完成签到,获得积分10
25秒前
26秒前
29秒前
落寞凌波发布了新的文献求助10
29秒前
29秒前
30秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029