亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

脂类学 失调 生物 肠道菌群 厚壁菌 微生物群 代谢组学 代谢组 拟杆菌 脂质代谢 微生物学 免疫学 生物信息学 细菌 生物化学 遗传学 16S核糖体RNA
作者
Yiyi Li,Hongmei Yan,Yao-Wei Zhang,Ping Fang,Lu Yu,Qianyu Li,Cuiting Liu,Yuwen Xie,Keli Chen,Feng Ye,Kai Wang,Longhua Chen,Yi Ding
出处
期刊:Frontiers in Cellular and Infection Microbiology [Frontiers Media]
卷期号:10 被引量:61
标识
DOI:10.3389/fcimb.2020.541178
摘要

Radiation enteritis (RE) is a common complication in cancer patients receiving radiotherapy. Although studies have shown the changes of this disease at clinical, pathological and other levels, the dynamic characteristics of local microbiome and metabolomics are hitherto unknown. We aimed to examine the multi-omics features of the gut microecosystem, determining the functional correlation between microbiome and lipid metabolites during RE activity. By delivering single high-dose irradiation, a RE mouse model was established. High-throughput16S rDNA sequencing and global lipidomics analysis were performed to examine microbial and lipidomic profile changes in the gut microecosystem. Spearman correlation analysis was used to determine the functional correlation between bacteria and metabolites. Clinical samples were collected to validate the above observations. During RE activity, the intestinal inflammation of the mice was confirmed by typical signs, symptoms, imaging findings and pathological evidences. 16S datasets revealed that localised irradiation dramatically altered the gut microbial composition, resulting in a decrease ratio of Bacteroidetes to Firmicutes. Lipidomics analysis indicated the remarkable lipidomic profile changes in enteric epithelial barrier, determining that glycerophospholipids metabolism was correlated to RE progression with the highest relevance. Spearman correlation analysis identified that 5 bacteria-metabolite pairs showed the most significant functional correlation in RE, including Alistipes-PC(36:0e), Bacteroides-DG(18:0/20:4), Dubosiella-PC(35:2), Eggerthellaceae-PC(35:6), Escherichia-Shigella-TG(18:2/18:2/20:4). These observations were partly confirmed in human specimens. Our study provided a comprehensive description of microbiota dysbiosis and lipid metabolic disorders in RE, suggesting strategies to change local microecosystem to relieve radiation injury and maintain homeostasis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
20秒前
28秒前
科研通AI5应助晚晚采纳,获得10
33秒前
jianghs发布了新的文献求助30
33秒前
袁钰琳完成签到 ,获得积分10
45秒前
49秒前
52秒前
晚晚完成签到,获得积分10
52秒前
晚晚发布了新的文献求助10
55秒前
59秒前
僦是卜够完成签到 ,获得积分10
1分钟前
徐悦月关注了科研通微信公众号
1分钟前
1分钟前
1分钟前
徐悦月发布了新的文献求助10
1分钟前
王志鹏发布了新的文献求助10
1分钟前
1分钟前
青木香发布了新的文献求助10
1分钟前
怡然的绮烟完成签到 ,获得积分10
1分钟前
青木香完成签到,获得积分10
1分钟前
Hello应助怡然的绮烟采纳,获得10
2分钟前
2分钟前
nolan完成签到 ,获得积分10
2分钟前
小蘑菇应助科研通管家采纳,获得10
2分钟前
kk完成签到 ,获得积分10
2分钟前
2分钟前
Elvira完成签到,获得积分10
2分钟前
先玩了玉发布了新的文献求助10
2分钟前
笨笨完成签到,获得积分10
3分钟前
vchen0621完成签到,获得积分0
3分钟前
certe完成签到,获得积分10
3分钟前
3分钟前
vchen0621发布了新的文献求助10
3分钟前
先玩了玉完成签到,获得积分10
3分钟前
俞无声完成签到 ,获得积分10
3分钟前
花凉发布了新的文献求助20
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
研友_VZG7GZ应助科研通管家采纳,获得10
4分钟前
4分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990031
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256334
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805146
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228