类有机物
体内
间质细胞
癌细胞
三维细胞培养
细胞生物学
细胞培养
微技术
癌症
舱室(船)
生物
化学
癌症研究
纳米技术
材料科学
生物技术
地质学
海洋学
遗传学
作者
Meng Horng Lee,Yohan Suryo Rahmanto,Gabriella C. Russo,Pei Hsun Wu,Daniele M. Gilkes,Ashley Kiemen,Tsutomu Miyamoto,Yu Yu,Mehran Habibi,Ie Ming Shih,Tian Li Wang,Denis Wirtz
标识
DOI:10.1101/2020.11.03.367334
摘要
Abstract Organoid cultures are widely used because they preserve many features of cancer cells in vivo . Here, we developed high-throughput oil-in-water droplet microtechnology to generate highly uniform, small-volume, multi-compartment organoids. Each organoid culture features a microenvironmental architecture that mimics both the basement membrane and stromal barriers. This matrix architecture, which allows accessing both proliferative and invasive features of cancer cells in a single platform, has profound effect on observed drug responsiveness and tumor progression that correlate well with in vivo and clinical outcomes. The method was tested on multiple types of cancer cells including primary cells and immortalized cell lines, and we determined our platform is suitable even for cells of poor organoid-forming ability. These new organoids also allow for direct orthotopic mouse implantation of cancer cells with unprecedented success.
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