EB1 protein alteration characterizes sporadic but not ulcerative colitis associated colorectal cancer

医学 结直肠癌 溃疡性结肠炎 家庭医学 内科学 癌症 普通外科 疾病
作者
Timo Gemoll,Sophie Louise Gisela Kollbeck,Karl Karstens,Gia G. Hò,Sonja Hartwig,Sarah Strohkamp,Katharina Schillo,Christoph Thorns,Martina Oberländer,Kathrin Kalies,Stefan Lehr,Jens K. Habermann
出处
期刊:Oncotarget [Impact Journals LLC]
卷期号:8 (33): 54939-54950 被引量:8
标识
DOI:10.18632/oncotarget.18978
摘要

// Timo Gemoll 1, * , Sophie L. Kollbeck 1, * , Karl F. Karstens 1 , Gia G. Hò 1 , Sonja Hartwig 2, 3 , Sarah Strohkamp 1 , Katharina Schillo 1 , Christoph Thorns 4 , Martina Oberländer 1 , Kathrin Kalies 5 , Stefan Lehr 2, 3 and Jens K. Habermann 1 1 Section for Translational Surgical Oncology and Biobanking, Department of Surgery, University of Lübeck and University Hospital Schleswig-Holstein, Campus Lübeck, D-23538 Lübeck, Germany 2 Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Düsseldorf, Leibniz Center for Diabetes Research, D-40225 Düsseldorf, Germany 3 German Center for Diabetes Research (DZD), D-85764 München-Neuherberg, Germany 4 Department of Pathology, University Hospital Schleswig-Holstein, Campus Lübeck, D-23538 Lübeck, Germany 5 Institute of Anatomy, University of Lübeck, D-23538 Lübeck, Germany * These authors contributed equally to this work Correspondence to: Timo Gemoll, email: Timo.Gemoll@uni-luebeck.de Jens K. Habermann, email: Jens.Habermann@uni-luebeck.de Keywords: ulcerative colitis associated colorectal cancer, EB1, two-dimensional gel electrophoresis, mass spectrometry, colorectal cancer Received: February 14, 2017      Accepted: June 17, 2017      Published: July 04, 2017 ABSTRACT Background: While carcinogenesis in Sporadic Colorectal Cancer (SCC) has been thoroughly studied, less is known about Ulcerative Colitis associated Colorectal Cancer (UCC). This study aimed to identify and validate differentially expressed proteins between clinical samples of SCC and UCC to elucidate new insights of UCC/SCC carcinogenesis and progression. Results: Multiplex-fluorescence two-dimensional gel electrophoresis (2-D DIGE) and mass spectrometry identified 67 proteoforms representing 43 distinct proteins. After analysis by Ingenuity Pathway Analysis ® (IPA), subsequent Western blot validation proofed the differential expression of Heat shock 27 kDA protein 1 (HSPB1) and Microtubule-associated protein R/EB family, member 1 (EB1) while the latter one showed also expression differences by immunohistochemistry. Materials and Methods: Fresh frozen tissue of UCC ( n = 10) matched with SCC ( n = 10) was investigated. Proteins of cancerous intestinal mucosal cells were obtained by Laser Capture Microdissection (LCM) and compared by 2-D DIGE. Significant spots were identified by mass spectrometry. After IPA, three proteins [EB1, HSPB1, and Annexin 5 (ANXA5)] were chosen for further validation by Western blotting and tissue microarray-based immunohistochemistry. Conclusions: This study identified significant differences in protein expression of colorectal carcinoma cells from UCC patients compared to patients with SCC. Particularly, EB1 was validated in an independent clinical cohort.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
清平道人完成签到,获得积分10
刚刚
刚刚
充电宝应助豆子采纳,获得10
1秒前
天天快乐应助李锐采纳,获得10
1秒前
呆萌安卉完成签到,获得积分10
1秒前
慕青应助幸福冰珍采纳,获得10
2秒前
NexusExplorer应助L112233采纳,获得10
2秒前
礼礼发布了新的文献求助10
2秒前
CXC发布了新的文献求助10
2秒前
Orange应助gy042876采纳,获得30
2秒前
路旁小白发布了新的文献求助10
2秒前
3秒前
令狐涵易发布了新的文献求助10
3秒前
天天完成签到,获得积分10
4秒前
包勇发布了新的文献求助30
4秒前
思源应助好奇宝宝采纳,获得10
4秒前
坚果发布了新的文献求助10
4秒前
Jason-1024发布了新的文献求助10
4秒前
5秒前
5秒前
Jemmy完成签到,获得积分10
6秒前
houfei发布了新的文献求助10
7秒前
Jiaxing完成签到,获得积分10
7秒前
卡卡西应助无限数据线采纳,获得20
8秒前
8秒前
量子星尘发布了新的文献求助10
9秒前
9秒前
郑昕玥发布了新的文献求助10
9秒前
乖猫要努力应助ahang采纳,获得10
10秒前
lulubeans应助1111采纳,获得20
10秒前
Jiaxing发布了新的文献求助10
10秒前
Muncy发布了新的文献求助10
10秒前
Li_华发布了新的文献求助10
11秒前
11秒前
11秒前
zhouxiuman完成签到,获得积分10
11秒前
12秒前
猜fing完成签到,获得积分10
12秒前
特种兵完成签到 ,获得积分10
12秒前
13秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971277
求助须知:如何正确求助?哪些是违规求助? 3516009
关于积分的说明 11180382
捐赠科研通 3251075
什么是DOI,文献DOI怎么找? 1795664
邀请新用户注册赠送积分活动 875988
科研通“疑难数据库(出版商)”最低求助积分说明 805209