EB1 protein alteration characterizes sporadic but not ulcerative colitis associated colorectal cancer

医学 结直肠癌 溃疡性结肠炎 家庭医学 内科学 癌症 普通外科 疾病
作者
Timo Gemoll,Sophie Louise Gisela Kollbeck,Karl Karstens,Gia G. Hò,Sonja Hartwig,Sarah Strohkamp,Katharina Schillo,Christoph Thorns,Martina Oberländer,Kathrin Kalies,Stefan Lehr,Jens K. Habermann
出处
期刊:Oncotarget [Impact Journals LLC]
卷期号:8 (33): 54939-54950 被引量:8
标识
DOI:10.18632/oncotarget.18978
摘要

// Timo Gemoll 1, * , Sophie L. Kollbeck 1, * , Karl F. Karstens 1 , Gia G. Hò 1 , Sonja Hartwig 2, 3 , Sarah Strohkamp 1 , Katharina Schillo 1 , Christoph Thorns 4 , Martina Oberländer 1 , Kathrin Kalies 5 , Stefan Lehr 2, 3 and Jens K. Habermann 1 1 Section for Translational Surgical Oncology and Biobanking, Department of Surgery, University of Lübeck and University Hospital Schleswig-Holstein, Campus Lübeck, D-23538 Lübeck, Germany 2 Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Düsseldorf, Leibniz Center for Diabetes Research, D-40225 Düsseldorf, Germany 3 German Center for Diabetes Research (DZD), D-85764 München-Neuherberg, Germany 4 Department of Pathology, University Hospital Schleswig-Holstein, Campus Lübeck, D-23538 Lübeck, Germany 5 Institute of Anatomy, University of Lübeck, D-23538 Lübeck, Germany * These authors contributed equally to this work Correspondence to: Timo Gemoll, email: Timo.Gemoll@uni-luebeck.de Jens K. Habermann, email: Jens.Habermann@uni-luebeck.de Keywords: ulcerative colitis associated colorectal cancer, EB1, two-dimensional gel electrophoresis, mass spectrometry, colorectal cancer Received: February 14, 2017      Accepted: June 17, 2017      Published: July 04, 2017 ABSTRACT Background: While carcinogenesis in Sporadic Colorectal Cancer (SCC) has been thoroughly studied, less is known about Ulcerative Colitis associated Colorectal Cancer (UCC). This study aimed to identify and validate differentially expressed proteins between clinical samples of SCC and UCC to elucidate new insights of UCC/SCC carcinogenesis and progression. Results: Multiplex-fluorescence two-dimensional gel electrophoresis (2-D DIGE) and mass spectrometry identified 67 proteoforms representing 43 distinct proteins. After analysis by Ingenuity Pathway Analysis ® (IPA), subsequent Western blot validation proofed the differential expression of Heat shock 27 kDA protein 1 (HSPB1) and Microtubule-associated protein R/EB family, member 1 (EB1) while the latter one showed also expression differences by immunohistochemistry. Materials and Methods: Fresh frozen tissue of UCC ( n = 10) matched with SCC ( n = 10) was investigated. Proteins of cancerous intestinal mucosal cells were obtained by Laser Capture Microdissection (LCM) and compared by 2-D DIGE. Significant spots were identified by mass spectrometry. After IPA, three proteins [EB1, HSPB1, and Annexin 5 (ANXA5)] were chosen for further validation by Western blotting and tissue microarray-based immunohistochemistry. Conclusions: This study identified significant differences in protein expression of colorectal carcinoma cells from UCC patients compared to patients with SCC. Particularly, EB1 was validated in an independent clinical cohort.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
青水完成签到 ,获得积分10
4秒前
量子星尘发布了新的文献求助10
4秒前
4秒前
冷酷尔琴完成签到,获得积分10
4秒前
onevip完成签到,获得积分0
6秒前
小莫完成签到 ,获得积分10
8秒前
17秒前
theseus完成签到,获得积分10
18秒前
胡楠完成签到,获得积分10
20秒前
北国雪未消完成签到 ,获得积分10
21秒前
李振博完成签到 ,获得积分10
21秒前
31秒前
雪妮完成签到 ,获得积分10
34秒前
松松发布了新的文献求助20
37秒前
37秒前
iwsaml完成签到 ,获得积分10
37秒前
Caden完成签到 ,获得积分10
40秒前
xmhxpz完成签到,获得积分10
41秒前
was_3完成签到,获得积分10
41秒前
聪慧板凳完成签到,获得积分10
45秒前
51秒前
buerzi完成签到,获得积分10
51秒前
魁梧的盼望完成签到 ,获得积分10
53秒前
量子星尘发布了新的文献求助30
54秒前
57秒前
wzk完成签到,获得积分10
58秒前
称心翠容完成签到,获得积分10
1分钟前
LaixS完成签到,获得积分10
1分钟前
尊敬代亦发布了新的文献求助10
1分钟前
要笑cc完成签到,获得积分10
1分钟前
青珊发布了新的文献求助10
1分钟前
宣宣宣0733完成签到,获得积分10
1分钟前
俊逸吐司完成签到 ,获得积分10
1分钟前
ttxxcdx完成签到 ,获得积分10
1分钟前
胡质斌完成签到,获得积分10
1分钟前
充电宝应助科研通管家采纳,获得10
1分钟前
1分钟前
姚怜南完成签到,获得积分10
1分钟前
青珊完成签到,获得积分10
1分钟前
自觉石头完成签到 ,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038029
求助须知:如何正确求助?哪些是违规求助? 3575740
关于积分的说明 11373751
捐赠科研通 3305559
什么是DOI,文献DOI怎么找? 1819224
邀请新用户注册赠送积分活动 892652
科研通“疑难数据库(出版商)”最低求助积分说明 815022