平滑
刺猬信号通路
刺猬
化学
细胞生物学
生物化学
计算生物学
生物
信号转导
作者
Ao Hu,Meng Zhou,Bao‐Liang Song
出处
期刊:Methods in molecular biology
日期:2021-09-25
卷期号:: 27-36
被引量:4
标识
DOI:10.1007/978-1-0716-1701-4_3
摘要
Cholesterol modification (or cholesterylation) is a rare but important posttranslational lipid modification of proteins. So far, two proteins, namely Hedgehog and Smoothened, have been characterized to undergo cholesterylation. It is unknown whether other proteins are similarly modified. Here we present a protocol of a chemical biology method to analyze the Smoothened protein cholesterylation using an azido-conjugated cholesterol analog combined with click chemistry. This method can be applied to analyze Hedgehog cholesterylation, and be extended to identify novel cholesterylated proteins with minor changes.
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