A Practical Electrochemical Nanotool for Facile Quantification of Amino Acids in Single Cell

Abstract Though significant advances are made in the arena of single‐cell electroanalysis, quantification of intracellular amino acids of human cells remains unsolved. Exemplified by l ‐histidine ( l ‐His), this issue is addressed by a practical electrochemical nanotool synergizing the highly accessible nanopipette with commercially available synthetic DNAzyme. The fabricated nanotools are screened before operation of a single‐use manner, and the l ‐His‐provoked cleavage of the DNA molecules can be sensibly transduced by the ionic current rectification response, the intrinsic property of nanopipette governed by its interior surface charges. Regional distribution of cytosolic l ‐His level in human cells is electrochemically quantified for the first time, and time‐dependent drug treatment effects are further revealed. This work unveils the possibility of electrochemistry for quantification of cytosolic amino acids of a spatial‐ and time‐based manner and ultimately enables a better understanding of amino acid‐involved events in living cells.