The heparinase-linked differential time method allows detection of heparin potency in whole blood with high sensitivity and dynamic range

肝素 重复性 效力 全血 检出限 化学 抗凝血酶 抗凝剂 色谱法 凝血酶 药理学 生物医学工程 医学 免疫学 内科学 血小板 生物化学 体外
作者
Anran Zheng,Wei Zhang,Chao Li,Zhen Guo,Chuanyu Li,Changsong Zhang,Jia Yao,Zhiqi Zhang,Jinze Li,Shasha Zhao,Lianqun Zhou
出处
期刊:Biosensors and Bioelectronics [Elsevier]
卷期号:198: 113856-113856 被引量:6
标识
DOI:10.1016/j.bios.2021.113856
摘要

Anticoagulation therapy with heparin is an effective treatment against thrombosis. Heparin tends to cause spontaneous bleeding and requires regular monitoring during therapy. Most high-sensitivity heparin sensors have focused on the concentration detection in clarified buffer solution. However, the pharmacodynamics of heparin vary depending on individual patient or disease, while potency detection with high sensitivity and dynamic range outperforms concentration detection in clinical diagnosis. In this study, a novel heparinase-linked differential time (HLDT) method was established with a two-zone of Graphene modified Carbon (GR-C) sensor, which was utilized to evaluate heparin potency in whole blood. It was based on electrochemical measurement of clotting time shifting associated with presence or absence of heparinase. Heparinase inhibits the anticoagulant ability of heparin by forming a heparin-antithrombin-thrombin complex during coagulation. And the intensity and peak time of electrochemical current were associated with thrombin activity and clotting on the electrode. The results demonstrated that the sensor had high selectivity for heparin potency in 10 μL of whole blood with a detection limit of 0.1 U/mL, and the linear detection range was 0.1-5 U/mL. The coefficient of variation (CV) of the peak time was less than 5%, and linear correlation between the GR-C sensor and the TEG-5000 instrument was 0.987. Thus, the HLDT method has better clinical application due to its good repeatability, high sensitivity and wide range in heparin potency evaluation.
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