Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli

Enterotoxigenic Escherichia coli (ETEC) is the main bacterial cause of diarrhea among children in developing countries and of traveler's diarrhea. In this study, a mouse model was used to evaluate the effect of Bifidobacterium on alleviating diarrhea caused by ETEC. The results showed that B. breve FHNFQ23M3 and B. bifidum FSDJN7O5 could relieve the symptoms of diarrhea. Both strains significantly reduced the stool water content, restored the villi structure in the jejunum and ameliorated the fecal short-chain fatty acid (SCFA) content. In addition, B. breve FHNFQ23M3 restored body weight to the level before ETEC challenge and significantly reduced interferon-γ (IFN-γ), while B. bifidum FSDJN7O5 significantly improved interleukin (IL)-10. Furthermore, all the Bifidobacterium strains used in this study could significantly downregulate tumor necrosis factor-α (TNF-α) and restore the unbalanced gut microbiota, which had a high content of pathogenic Escherichia-Shigella and low content of Blautia and Clostridium innocuum groups due to ETEC. All the results proved that Bifidobacterium could be a potential probiotic for alleviating diarrhea from ETEC infection.