Dietary yeast culture facilitates the growth, immune response, digestive enzyme activity, intestinal microbiota and disease resistance against Vibrio harveyi of hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Aquaculture ResearchVolume 53, Issue 6 p. 2484-2500 ORIGINAL ARTICLE Dietary yeast culture facilitates the growth, immune response, digestive enzyme activity, intestinal microbiota and disease resistance against Vibrio harveyi of hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Qi Wang, Qi Wang Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China Bio-Form Biotechnology (Guangdong) Co., Ltd., Zhanjiang, ChinaSearch for more papers by this authorStephen Ayiku, Stephen Ayiku Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorHongyu Liu, Corresponding Author Hongyu Liu liuhyu@gdou.edu.cn orcid.org/0000-0002-8111-3941 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China Correspondence Hongyu Liu, Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China. Email: liuhyu@gdou.edu.cnSearch for more papers by this authorBeiping Tan, Beiping Tan Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorXiaohui Dong, Xiaohui Dong orcid.org/0000-0002-0368-4837 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorShuyan Chi, Shuyan Chi orcid.org/0000-0002-8753-8318 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorQihui Yang, Qihui Yang orcid.org/0000-0001-9107-2541 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorShuang Zhang, Shuang Zhang Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorWenhao Zhou, Wenhao Zhou Beijing Enhalor International Tech Co., Ltd., Beijing, ChinaSearch for more papers by this author Qi Wang, Qi Wang Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China Bio-Form Biotechnology (Guangdong) Co., Ltd., Zhanjiang, ChinaSearch for more papers by this authorStephen Ayiku, Stephen Ayiku Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorHongyu Liu, Corresponding Author Hongyu Liu liuhyu@gdou.edu.cn orcid.org/0000-0002-8111-3941 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China Correspondence Hongyu Liu, Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China. Email: liuhyu@gdou.edu.cnSearch for more papers by this authorBeiping Tan, Beiping Tan Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorXiaohui Dong, Xiaohui Dong orcid.org/0000-0002-0368-4837 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorShuyan Chi, Shuyan Chi orcid.org/0000-0002-8753-8318 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorQihui Yang, Qihui Yang orcid.org/0000-0001-9107-2541 Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorShuang Zhang, Shuang Zhang Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, ChinaSearch for more papers by this authorWenhao Zhou, Wenhao Zhou Beijing Enhalor International Tech Co., Ltd., Beijing, ChinaSearch for more papers by this author First published: 27 January 2022 https://doi.org/10.1111/are.15766 Qi Wang and Stephen Ayiku Co-first author and contributed equally to the study. Funding information: This work was supported by the National Key R&D Program of China (2019YFD0900200), National Science Foundation of China (No. 31772864), Guangdong province enterprise sci-tech commissioner (GDKTP2020017800) and Natural Science Foundation of Guangdong Province (2018A030313154 & 2020A1515011129). Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The present study aimed to investigate the effect of yeast culture (YC) supplementation at different levels (0%, 2% and 4%) on the growth, immune response, digestive enzyme activity, intestinal morphology and microbiota of hybrid grouper. After 56 days feeding trial, the results showed that the weight gain rate, specific growth rate, survival rate and protein efficiency rate of hybrid grouper were significantly improved, and the feed conversion ratio of hybrid grouper in the 4% group (p < 0.05) was significantly reduced. The serum total protein, acid phosphatase, alkaline phosphatase and lysozyme of hybrid grouper in the 4% group were significantly higher than those in the control group, while the alanine transaminase, aspartate transaminase and malondialdehyde were significantly lower than those in the control group (p < 0.05). YC treatment significantly enhanced the trypsin, lipase and amylase activities (p < 0.05). Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes constituted the dominant phylum in grouper intestine. The relative abundance of Sphingomonas, Stenotrophomonas and Phyllobacterium in the YC groups was higher than that in the control group. The length and width of intestinal villi of grouper fed with YC diets were improved (p < 0.05). The protection against V. harveyi challenge in hybrid grouper was enhanced after YC treatment group, and the hybrid groupers fed with 4% YC diets experienced the highest cumulative survival rate of 67%. All these results suggested that the supplementation of 4% YC diets improved the growth performance, digestive enzyme activity, intestinal morphology, microbiota and disease resistance against V. harveyi of hybrid grouper. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Volume53, Issue6April 2022Pages 2484-2500 RelatedInformation