细胞凋亡
膜联蛋白
细胞毒性
细胞周期
细胞生长
分子生物学
流式细胞术
细胞周期检查点
免疫印迹
体外
细胞毒性T细胞
圆周率
化学
程序性细胞死亡
细胞
生物
细胞生物学
生物化学
基因
作者
Shixiu Feng,Min Zhang,Jing Xu,Yinming Hu
标识
DOI:10.1080/14786419.2016.1160234
摘要
Prisconnatanones A (Priscon-A) is a rare tetrahydroanthraquinone isolated from herbal Prismatomeris connate. In this study, we examine its anti-tumour activity on human laryngocarcinoma HEp-2 cells in vitro. The CCK-8 assay was performed to evaluate its cytotoxicity. Cell cycle and apoptosis were analysed using flow cytometric analysis. Here, we showed Priscon-A inhibited the proliferation of HEp-2 cells in a dose-dependent manner, and at 5 μM it almost completely inhibited cell growth. Its cytotoxicity was associated with the cell cycle arrest at G2/M phase. The Annexin V-FITC/PI binding assay showed that the cell death induced by Priscon-A was associated with apoptosis. And, western blot analysis revealed that the levels of the apoptosis protein, cleaved caspase-3, PARP, p21 and Bax protein increased, while the level of anti-apoptosis protein Bcl-2 decreased.. These data demonstrated that Priscon-A significantly inhibited HEp-2 cell growth, induced the cell cycle arrest at the G2/M phase and efficiently induced cell apoptosis.
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