弹性蛋白酶
化学
中性粒细胞弹性蛋白酶
生物化学
粘液
弹性蛋白
胰弹性蛋白酶
DNA
分子生物学
酶抑制剂
酶
琼脂糖
生物
免疫学
生态学
遗传学
炎症
作者
Didier Belorgey,Joseph G. Bieth
出处
期刊:FEBS Letters
[Wiley]
日期:1995-03-20
卷期号:361 (2-3): 265-268
被引量:36
标识
DOI:10.1016/0014-5793(95)00173-7
摘要
DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. DNA is a potent hyperbolic inhibitor of elastase. The polynucleotide-enzyme complex is partially active on synthetic substrates and on elastin. DNA strongly increases kdiss and Ki for the inhibition of elastase by mucus proteinase inhibitor [formula: see text] The above effects are all salt-dependent. At physiological ionic strength, DNA is a potent inhibitor of the elastolytic activity of elastase and increases kdiss and Ki for the elastase-mucus proteinase inhibitor interaction 160-fold and 100-fold, respectively.
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