Fetal testosterone insufficiency and abnormal proliferation of Leydig cells and gonocytes in rats exposed to di(n-butyl) phthalate

生殖细胞 邻苯二甲酸盐 间质细胞 内分泌学 内科学 胎儿 睾酮(贴片) 邻苯二甲酸二丁酯 睾丸 生物 男科 医学 化学 怀孕 支持细胞 精子发生 激素 遗传学 促黄体激素 有机化学
作者
Eve Mylchreest,Madhabananda Sar,Duncan G. Wallace,Paul M.D. Foster
出处
期刊:Reproductive Toxicology [Elsevier]
卷期号:16 (1): 19-28 被引量:316
标识
DOI:10.1016/s0890-6238(01)00201-5
摘要

Adult male rats previously exposed on gestation days (GD) 12–21 to di(n-butyl) phthalate (DBP) have reproductive tract malformations, particularly agenesis of the epididymis, decreased sperm production, and Leydig cell hyperplasia and adenomas. Although similar effects are produced by the potent androgen receptor (AR) antagonist flutamide and are indicative of disruption of male sexual differentiation via an antiandrogenic mechanism, DBP is not an AR antagonist. The purpose of the study was to determine whether DBP causes pathologic changes and alterations in androgen status in the testis during the prenatal period of male reproductive tract differentiation. Pregnant CD rats were given corn oil, DBP (500 mg/kg/day), or flutamide (100 mg/kg/day) p.o. on GD 12–21. At GD 16–21, DBP caused hyperplasia of Leydig cells, many of which were 3β-hydroxysteroid dehydrogenase- and/or AR-positive. Focal areas of hyperplasia had increased numbers of Leydig cells positive for proliferating cell nuclear antigen (PCNA). At GD 21, testis atrophy was apparent, seminiferous cords in DBP-exposed fetuses were enlarged and contained multinucleated gonocytes that, unlike controls, were PCNA-positive. DBP, but not flutamide, markedly decreased testicular testosterone levels at GD 18 and 21. Fewer epididymal ducts and reduced AR staining in some ducts were evident with DBP treatment, whereas decreased overall AR staining was seen with flutamide in the presence of mild Leydig cell hyperplasia. Leydig cell proliferation is likely a compensatory mechanism to increase testicular steroidogenesis triggered by testosterone insufficiency. The overall decrease in androgen concentration is not corrected and results in reproductive tract malformations. The multinuclearity and proliferation of gonocytes suggests an underlying Sertoli cell dysfunction.
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