化学
体外
钌
体内
单体
吩嗪
纤维
蛋白质聚集
钌红
生物物理学
立体化学
生物化学
有机化学
催化作用
生物技术
生物
钙
聚合物
作者
Nathan P. Cook,Kiri Kilpatrick,Laura Segatori,Ángel A. Martí
摘要
Protein aggregation is the hallmark of a number of neurodegenerative diseases including Parkinson's and Huntington's diseases. There is a significant interest in understanding the molecular mechanisms involved in the self-association and fibrillization of monomeric soluble proteins into insoluble deposits in vivo and in vitro. Probes with novel properties, such as red-shifted emission, large Stokes shifts, and high photostability, are desirable for a variety of protein aggregation studies. To respond to the increasing need for aggregation–responsive compounds suitable to cellular studies, we present a ruthenium(II) dipyridophenazine derivative, [Ru(phen)2dppz]2+ (phen =1,10-phenanthroline, dppz = dipyrido[3,2-a:2′.3′-c]phenazine), to study aggregation of α-synuclein (αS), which is associated with the development of Parkinson's disease. We demonstrated the use of [Ru(phen)2dppz]2+ to monitor αS fibril formation in real-time and to detect and quantify αS aggregates in neuroglioma cells, thereby providing a novel molecular tool to study protein deposition diseases in vitro and in vivo.
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