Comparison of Alternative Splicing Junction Detection Tools Using RNASeq Data

Background: Alternative splicing (AS) is a posttranscriptional process that produces different transcripts from the same gene and is important to produce diverse protein products in response to environmental stimuli. AS occurs at specific sites on the mRNA sequence, some of which have been defined. Multiple bioinformatics tools have been developed to detect AS from experimental data. Keywords: Alternative splicing, Splice junctions detection, RNA-seq, Spliceosome, Read-split-run, Read-split-walk.