Cytochemical Localization of Polysaccharides in Dendrobium officinale and the Involvement of DoCSLA6 in the Synthesis of Mannan Polysaccharides

多糖 甘露聚糖 单糖 葡聚糖 生物化学 淀粉 细胞壁 化学 纤维素 甘露糖 葡甘露聚糖 鼠李糖 生物 甲壳素 碳水化合物
作者
Chunmei He,Kunlin Wu,Jianxia Zhang,Xuncheng Liu,Songjun Zeng,Zhenming Yu,Xinghua Zhang,Jaime A. Teixeira da Silva,Renhua Deng,Jian‐Wen Tan,Jian‐Ping Luo,Jun Duan
出处
期刊:Frontiers in Plant Science [Frontiers Media SA]
卷期号:8 被引量:32
标识
DOI:10.3389/fpls.2017.00173
摘要

Dendrobium officinale is a precious traditional Chinese medicinal plant because of its abundant polysaccharides found in stems. We determined the composition of water-soluble polysaccharides and starch content in D. officinale stems. The extracted water-soluble polysaccharide content was as high as 35% (w/w). Analysis of the composition of monosaccharides showed that the water-soluble polysaccharides were dominated by mannose, to a lesser extent glucose, and a small amount of galactose, in a molar ratio of 223:48:1. Although starch was also found, its content was less than 10%. This result indicated that the major polysaccharides in D. officinale stems were non-starch polysaccharides, which might be mannan polysaccharides. The polysaccharides formed granules and were stored in plastids similar to starch grains, were localized in D. officinale stems by semi-thin and ultrathin sections. CELLULOSE SYNTHASE-LIKE A (CSLA) family members encode mannan synthases that catalyze the formation of mannan polysaccharides. To determine whether the CSLA gene from D. officinale was responsible for the synthesis of mannan polysaccharides, 35S:DoCSLA6 transgenic lines were generated and characterized. Our results suggest that the CSLA family genes from D. officinale play an important role in the biosynthesis of mannan polysaccharides.
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