微流控
微流控芯片
实验室晶片
炸薯条
枚举
化学
纳米技术
计算机硬件
吞吐量
计算机科学
工艺工程
工程类
材料科学
数学
电信
组合数学
无线
作者
Xiaolin Wu,Bingliang Xie,Yuxin Qiao,Shuai Yuan,Wenbin Du
标识
DOI:10.1021/acs.analchem.3c04889
摘要
This work describes μMET, a novel microfluidic device for precise microbial enumeration tests (MET), essential in pharmaceutical, cosmetic, and food industries for ensuring microbiological safety standards. The μMET chip, comprising two hydrophobic glass plates, features a 15-μm deep μMET chamber enhanced by nanopillars and air supply units, facilitating both immediate and growth-dependent MET. Experimental results, with E. coli as a model bacterium, demonstrate that μMET provides counting linearity that outperforms traditional hemocytometers. The chip's design mitigates challenges like evaporation and ensures high-resolution imaging, making it a cost-effective and reusable alternative to conventional methods. Notably, bright-field μMET eliminates the need for fluorescent staining, streamlining operations with deep-learning algorithms for bacterial counts. Furthermore, we have developed a high-parallel μMET chip featuring 16 counting chambers, enhancing throughput and accommodating immediate and growth-dependent MET approaches. Its innovative design and adaptability render the μMET chip as a valuable tool for microbiology, medicine, and industry applications.
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