纳米传感器
荧光
表面改性
多巴胺
生物传感器
纳米技术
生物相容性
乙二醇
化学
生物物理学
PEG比率
材料科学
有机化学
神经科学
生物
物理
物理化学
财务
量子力学
经济
作者
Chen Ma,Jennifer M. Mohr,Günter Lauer,Justus T. Metternich,Krisztian Neutsch,Tim Ziebarth,Andreas Reiner,Sebastian Kruss
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-02-12
卷期号:24 (7): 2400-2407
被引量:2
标识
DOI:10.1021/acs.nanolett.3c05082
摘要
Neurotransmitters are important signaling molecules in the brain and are relevant in many diseases. Measuring them with high spatial and temporal resolutions in biological systems is challenging. Here, we develop a ratiometric fluorescent sensor/probe for catecholamine neurotransmitters on the basis of near-infrared (NIR) semiconducting single wall carbon nanotubes (SWCNTs). Phenylboronic acid (PBA)-based quantum defects are incorporated into them to interact selectively with catechol moieties. These PBA-SWCNTs are further modified with poly(ethylene glycol) phospholipids (PEG-PL) for biocompatibility. Catecholamines, including dopamine, do not affect the intrinsic E11 fluorescence (990 nm) of these (PEG-PL-PBA-SWCNT) sensors. In contrast, the defect-related E11* emission (1130 nm) decreases by up to 35%. Furthermore, this dual functionalization allows tuning selectivity by changing the charge of the PEG polymer. These sensors are not taken up by cells, which is beneficial for extracellular imaging, and they are functional in brain slices. In summary, we use dual functionalization of SWCNTs to create a ratiometric biosensor for dopamine.
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