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Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes

血淋巴 生物 埃及伊蚊 蛋白质组 蛋白质组学 先天免疫系统 蛋白酵素 丝氨酸蛋白酶 免疫系统 血粉 生物化学 动物 蛋白酶 免疫学 生态学 幼虫 基因
作者
Patrícia H. Alvarenga,Thiago Luiz Alves e Silva,Motoshi Suzuki,Glenn Nardone,Pedro Cecílio,Joel Vega-Rodríguez,José M. C. Ribeiro,John F. Andersen
出处
期刊:Journal of Proteome Research [American Chemical Society]
卷期号:23 (4): 1471-1487
标识
DOI:10.1021/acs.jproteome.3c00918
摘要

In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1403 proteins were identified, out of which 447 of them were predicted to be extracellular. In both sexes, almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundances (based on their intensity-based absolute quantification, iBAQ) were 37.9 and 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues were identified, with almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, such as odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of A. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on hemolymph composition.
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