动物双歧杆菌
生物
食品科学
双歧杆菌
微生物学
生物化学
发酵
乳酸菌
作者
Zongcai Zhang,Baoshu Yin,Fei Liu,Wei Zhou,Ming-Jye Wang,Zhi Chang,Junping Zhou,Maoxing Yue,Junxia Chen,Zhen Feng
标识
DOI:10.1093/jambio/lxae022
摘要
Abstract Aims During fermentation, the accumulation of acidic products can induce media acidification, which restrains the growth of Bifidobacterium animalis subsp. lactis Bb12 (Bb12). This study investigated the nutrient consumption patterns of Bb12 under acid stress and effects of specific nutrients on the acid resistance of Bb12. Methods and Results Bb12 was cultured in chemically defined medium (CDM) at different initial pH values. Nutrient consumption patterns were analyzed in CDM at pH 5.3, 5.7 and 6.7. The patterns varied with pH: Asp+Asn had the highest consumption rate at pH 5.3 and 5.7, while Ala was predominant at pH 6.7. Regardless of the pH levels (5.3, 5.7, or 6.7), ascorbic acid, adenine and Fe2+ were vitamins, nucleobases and metal ions with the highest consumption rates, respectively. Nutrients whose consumption rates exceeded 50% were added individually in CDM at pH 5.3, 5.7 and 6.7. It was demonstrated that only some of them could promote the growth of Bb12. Mixed nutrients which could promote the growth of Bb12 were added to three different CDM. In CDM at pH 5.3, 5.7 and 6.7, it was found that the viable cell count of Bb12 was the highest after adding mixed nutrients, which were 8.87, 9.02 and 9.10 log CFU mL−1, respectively. Conclusions The findings suggest that the initial pH of the culture medium affects the nutrient consumption patterns of Bb12. Specific nutrients can enhance the growth of Bb12 under acidic conditions and increase its acid resistance.
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