生物
翻译(生物学)
遗传学
进化生物学
真核翻译
计算生物学
基因
信使核糖核酸
作者
Tai‐Hsi Wu,Yaru Li,Kai-Jyun Chang,Jhen-Cheng Fang,Daisuke Urano,Ming-Jung Liu
标识
DOI:10.1101/gr.278100.123
摘要
mRNA translation relies on identifying translation initiation sites (TISs) in mRNAs. Alternative TISs are prevalent across plant transcriptomes, but the mechanisms for their recognition are unclear. Using ribosome profiling and machine learning, we developed models for predicting alternative TISs in the tomato ( Solanum lycopersicum ). Distinct feature sets were predictive of AUG and nonAUG TISs in 5′ untranslated regions and coding sequences, including a novel CU-rich sequence that promoted plant TIS activity, a translational enhancer found across dicots and monocots, and humans and viruses. Our results elucidate the mechanistic and evolutionary basis of TIS recognition, whereby cis -regulatory RNA signatures affect start site selection. The TIS prediction model provides global estimates of TISs to discover neglected protein-coding genes across plant genomes. The prevalence of cis -regulatory signatures across plant species, humans, and viruses suggests their broad and critical roles in reprogramming the translational landscape.
科研通智能强力驱动
Strongly Powered by AbleSci AI