Diagnostic Accuracy of

Edited MRS technique such as MEshcher-GArwood Point RESolved Spectroscopy (MEGA-PRESS) can determine isocitrate dehydrogenase mutation (IDH) mutation status in patients with gliomas but its accuracy in assessing glioma grade has not yet been formally evaluated. To evaluate the diagnostic accuracy of metabolites such as lactate obtained from the PRESS and MEGA-PRESS sequences in the preoperative grading of glioma. To assess the prognostic value of those metabolite ratios in the overall survival of patients with gliomas. Prospective. Sixty-nine subjects with gliomas (16 grade 2, 21 grade 3, and 32 grade 4). Mean age was 50.5 ± 16.7 years; 38 were male and 31 were female. 3 T/MEGA-PRESS, PRESS. Single voxel PRESS and MEGA-PRESS spectra were obtained from tumors in patients undergoing preoperative MRI. Several tumor metabolites were measured from the PRESS, MEGA-PRESS edit-off, and difference spectra using LCModel (Linear Combination of Model Spectra) software. Diagnosis and glioma grading was done using the World Health Organization (WHO) 2016 classification. Overall survival was assessed. Diagnostic accuracy was measured using receiver-operating characteristic (ROC) curve. Univariate and multivariate Cox proportional hazards modeling was used for the assessment of prognostic factors for time to death. In the differentiation between low- vs. high-grade gliomas, tCr/tCho ratios obtained from PRESS and MEGA-PRESS sequences had similar accuracies (area under the ROC curves [AUCs] = 0.71) while Lac/NAA from PRESS had a lower accuracy (AUC = 0.65). The presence of a detectable 2-hydroxyglutarate peak on the difference spectrum was a favorable prognostic factor in univariate analysis (hazard ratio = 0.25, 95% confidence interval: 0.08-0.83). No other metabolite was found to be a significant prognostic factor in univariate and multivariate analyses. Edited MRS can be used to detect metabolites which can help in the preoperative grading of gliomas and in determination of the overall survival. A separate PRESS acquisition is needed for lactate quantification. Gliomas are brain tumors that vary in severity. This study explored the use of two advanced MR spectroscopy techniques (PRESS and MEGA-PRESS) in detecting tumor metabolites. The authors found that both techniques' choline/creatine ratio showed moderate accuracy in identifying high-grade gliomas. Lactate was better revealed with the PRESS technique and was associated with high-grade gliomas. They confirmed that the MEGA-PRESS technique allowed additional detection of 2-hydroxyglutarate in IDH-mutant gliomas, which was linked to better survival. These findings emphasize that advanced MR spectroscopy can extract metabolic information time-efficiently, which can be used to improve the preoperative diagnosis of patients with gliomas. 1 TECHNICAL EFFICACY: Stage 2.