单克隆抗体
分子生物学
生物
抗体
免疫球蛋白轻链
病毒学
免疫印迹
直接荧光抗体
核蛋白
病毒
免疫学
基因
生物化学
作者
Shyam Kokkattunivarthil Uthaman,Min-Seok Jang,Kyoung-hui Kong,Myung‐Joo Oh,Wi‐Sik Kim
出处
期刊:Monoclonal antibodies in immunodiagnosis and immunotherapy
[Mary Ann Liebert]
日期:2023-04-01
卷期号:42 (2): 53-58
标识
DOI:10.1089/mab.2022.0040
摘要
Hirame novirhabdovirus (HIRRV) is a significant viral pathogen of Japanese flounder (Paralichthys olivaceus). In this study, seven monoclonal antibodies (mAbs) against HIRRV (isolate CA-9703) were produced and characterized. Three mAbs (1B3, 5G6, and 36D3) were able to recognize nucleoprotein (N) (42 kDa) and four mAbs (11-2D9, 15-1G9, 17F11, and 24-1C6) recognized matrix (M) protein (24 kDa) of HIRRV. Western blot, Enzyme-linked immunosorbent assay, and indirect fluorescent antibody technique (IFAT) results indicated that the developed mAbs were specific to HIRRV without any cross-reactivity against other different fish viruses and epithelioma papulosum cyprini cells. All the mAbs comprised IgG1 heavy chain and κ light chain except 5G6, which has a heavy chain of IgG2a class. These mAbs can be very useful in development of immunodiagnosis of HIRRV infection.
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