Diacylglycerol acyltransferase (DGAT) in Crangon crangon and Pandalus montagui (Decapoda, Caridea) - Implications for lipid storage capacities and life history traits

真虾类 十足目 生物 褐虾 动物 渔业 甲壳动物
作者
Marie Koch,Jan Tebben,Reinhard Saborowski
出处
期刊:Comparative Biochemistry and Physiology B [Elsevier]
卷期号:268: 110878-110878 被引量:2
标识
DOI:10.1016/j.cbpb.2023.110878
摘要

Lipids play essential roles in cell-structuring, cell-signaling, and as efficient metabolic energy stores. Lipid storage capacities determine life history traits of organisms and, thus, their ecological function. Among storage lipids, triacylglycerols (TAGs) are widespread in marine invertebrates. However, abilities to accumulate TAGs can vary even between closely related species, such as the caridean shrimps Crangon crangon and Pandalus montagui. The first species shows low TAG levels throughout the year in the main storage organ, the midgut gland, while the latter accumulates high TAG-levels, peaking in summer. TAGs synthesis is facilitated by the terminal step of the Kennedy-pathway, where the enzyme diacylglycerol-acyltransferase (DGAT) catalyzes the esterification of diacylglycerols with activated fatty acids. We investigated DGAT activity in the midgut gland using a fluorescent enzyme assay. Sequence information was extracted from whole transcriptome shotgun assembly data, that was publicly available on NCBI, and catalytic properties were deduced from molecular structure analysis. C. crangon showed significantly lower TAG synthesis rates than P. montagui, which explains the native TAG levels. Transcriptome data yielded several isoforms of DGAT enzymes in both species. C. crangon DGAT showed point mutations, which are capable of obstructing the catalytic capacity. The consequences are limited starvation resistance and, thus, presumably restricting C. crangon to a habitat with year-round sufficient food. In contrast, higher TAG synthesis rates presumably enable P. montagui to extend into northern subarctic habitats with limited food availability in winter. Moreover, the limited TAG synthesis and accumulation in the midgut gland may force C. crangon to direct energy into the ovaries, which results in multiple spawnings.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
xbchen完成签到,获得积分10
1秒前
3秒前
嗯哼应助Onlyyou采纳,获得20
5秒前
6秒前
科研通AI2S应助深情的巧蕊采纳,获得10
6秒前
7秒前
Lisby发布了新的文献求助10
7秒前
7秒前
8秒前
kjding发布了新的文献求助10
8秒前
科研通AI2S应助森林木采纳,获得10
9秒前
10秒前
shunlibiye发布了新的文献求助10
12秒前
13秒前
琥珀川发布了新的文献求助10
13秒前
二十八化生完成签到 ,获得积分10
13秒前
科研通AI2S应助XYZ采纳,获得30
13秒前
15秒前
17秒前
复杂的一笑关注了科研通微信公众号
20秒前
lapidary完成签到,获得积分10
21秒前
xuyirong完成签到,获得积分10
22秒前
23秒前
23秒前
醉生梦死完成签到,获得积分10
24秒前
25秒前
25秒前
26秒前
深情安青应助万卷神采纳,获得10
26秒前
27秒前
28秒前
lauchan54发布了新的文献求助10
28秒前
醉生梦死发布了新的文献求助10
28秒前
天高任鸟飞完成签到,获得积分10
29秒前
29秒前
LHL发布了新的文献求助10
30秒前
墨点完成签到 ,获得积分10
30秒前
ma发布了新的文献求助10
30秒前
华仔应助一二三四五采纳,获得10
30秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3256496
求助须知:如何正确求助?哪些是违规求助? 2898727
关于积分的说明 8301929
捐赠科研通 2567817
什么是DOI,文献DOI怎么找? 1394748
科研通“疑难数据库(出版商)”最低求助积分说明 652913
邀请新用户注册赠送积分活动 630602