Diacylglycerol acyltransferase (DGAT) in Crangon crangon and Pandalus montagui (Decapoda, Caridea) - Implications for lipid storage capacities and life history traits

真虾类 十足目 生物 褐虾 动物 渔业 甲壳动物
作者
Marie Koch,Jan Tebben,Reinhard Saborowski
出处
期刊:Comparative Biochemistry and Physiology B [Elsevier BV]
卷期号:268: 110878-110878 被引量:2
标识
DOI:10.1016/j.cbpb.2023.110878
摘要

Lipids play essential roles in cell-structuring, cell-signaling, and as efficient metabolic energy stores. Lipid storage capacities determine life history traits of organisms and, thus, their ecological function. Among storage lipids, triacylglycerols (TAGs) are widespread in marine invertebrates. However, abilities to accumulate TAGs can vary even between closely related species, such as the caridean shrimps Crangon crangon and Pandalus montagui. The first species shows low TAG levels throughout the year in the main storage organ, the midgut gland, while the latter accumulates high TAG-levels, peaking in summer. TAGs synthesis is facilitated by the terminal step of the Kennedy-pathway, where the enzyme diacylglycerol-acyltransferase (DGAT) catalyzes the esterification of diacylglycerols with activated fatty acids. We investigated DGAT activity in the midgut gland using a fluorescent enzyme assay. Sequence information was extracted from whole transcriptome shotgun assembly data, that was publicly available on NCBI, and catalytic properties were deduced from molecular structure analysis. C. crangon showed significantly lower TAG synthesis rates than P. montagui, which explains the native TAG levels. Transcriptome data yielded several isoforms of DGAT enzymes in both species. C. crangon DGAT showed point mutations, which are capable of obstructing the catalytic capacity. The consequences are limited starvation resistance and, thus, presumably restricting C. crangon to a habitat with year-round sufficient food. In contrast, higher TAG synthesis rates presumably enable P. montagui to extend into northern subarctic habitats with limited food availability in winter. Moreover, the limited TAG synthesis and accumulation in the midgut gland may force C. crangon to direct energy into the ovaries, which results in multiple spawnings.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
dmm完成签到 ,获得积分10
刚刚
佳期如梦完成签到 ,获得积分10
2秒前
展会恩完成签到,获得积分10
2秒前
干将莫邪完成签到,获得积分10
3秒前
执着夏岚完成签到 ,获得积分10
6秒前
6秒前
9秒前
lhn完成签到 ,获得积分10
9秒前
饱满烙完成签到 ,获得积分10
10秒前
菓小柒完成签到 ,获得积分10
10秒前
12秒前
13秒前
hongxing liu发布了新的文献求助10
18秒前
Dongjie发布了新的文献求助10
20秒前
杨永佳666完成签到 ,获得积分10
21秒前
Lisztan完成签到,获得积分10
24秒前
Tysonqu完成签到,获得积分10
24秒前
萤火虫完成签到,获得积分10
25秒前
饱满的新之完成签到 ,获得积分10
27秒前
songyu完成签到,获得积分10
28秒前
28秒前
黑苹果完成签到,获得积分10
30秒前
孙总完成签到,获得积分10
31秒前
健壮仙人掌完成签到,获得积分10
34秒前
鲸鱼打滚完成签到 ,获得积分10
35秒前
传奇3应助科研通管家采纳,获得10
38秒前
hongxing liu完成签到,获得积分10
40秒前
紫金之巅完成签到 ,获得积分10
41秒前
快乐的90后fjk完成签到 ,获得积分10
42秒前
漂亮的忆文完成签到,获得积分10
46秒前
蜗牛完成签到 ,获得积分10
48秒前
科研通AI2S应助hongxing liu采纳,获得10
51秒前
haochi完成签到,获得积分10
54秒前
Gavin完成签到,获得积分10
54秒前
fatcat完成签到,获得积分10
57秒前
qianci2009完成签到,获得积分10
58秒前
徐旖旎完成签到,获得积分10
1分钟前
1分钟前
光亮的自行车完成签到,获得积分0
1分钟前
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968559
求助须知:如何正确求助?哪些是违规求助? 3513358
关于积分的说明 11167370
捐赠科研通 3248804
什么是DOI,文献DOI怎么找? 1794465
邀请新用户注册赠送积分活动 875116
科研通“疑难数据库(出版商)”最低求助积分说明 804664