Tag‐free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition

适体 检出限 指数富集配体系统进化 化学 荧光染料 污染 线性范围 分析化学(期刊) 核化学 色谱法 实时聚合酶链反应 核糖核酸 分子生物学 生物化学 基因 生物 生态学 有机化学
作者
Jiangxiong Zhu,Hao Yin,Sisi Zheng,Hong Yu,Linnan Yang,Lumei Wang,Xueqing Geng,Yun Deng
出处
期刊:Journal of the Science of Food and Agriculture [Wiley]
标识
DOI:10.1002/jsfa.13631
摘要

Abstract BACKGROUND In response to growing concerns regarding heavy metal contamination in food, particularly chromium (Cr)(VI) contamination, this study presented a simple, sensitive and practical method for Cr(VI) detection. RESULTS A magnetic separation‐based capture–exponential enrichment ligand system evolution (SELEX) method was used to identify and characterize DNA aptamers with a high affinity for Cr(VI). An aptamer, Cr‐15, with a dissociation constant ( K d ) of 4.42 ± 0.44 μmol L −1 was obtained after only eight rounds of selection. Further innovative methods combining molecular docking, dynamic simulation and thermodynamic analysis revealed that CrO 4 2− could bind to the 19th and 20th guanine bases of Cr‐15 via hydrogen bonds. Crucially, a label‐free fluorometric aptasensor based on SYBR Green I was successfully constructed to detect CrO 4 2− , achieving a linear detection range of 60–300 nmol L −1 with a lower limit of detection of 44.31 nmol L −1 . Additionally, this aptasensor was able to quantitatively detect CrO 4 2− in grapes and broccoli within 40 min, with spike recovery rates ranging from 89.22% to 108.05%. The designed fluorometric aptasensor exhibited high selectivity and could detect CrO 4 2− in real samples without sample processing or target pre‐enrichment. CONCLUSION The aptasensor demonstrated its potential as a reliable tool for monitoring Cr(VI) contamination in fruit and vegetable products. © 2024 Society of Chemical Industry.
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