Characterization of two novel highly active glycoside hydrolase family 53 endo-1,4-β-galactanases and their synergism with other carbohydrases in plant polysaccharide decomposition

半乳糖 糖苷水解酶 化学 水解 多糖 纤维素酶 水解酶 糖苷 生物化学 立体化学
作者
Yurou Zhang,Yun Liu,Chunxiu Zeng,Yingyu Shu,Xiaoru Wang,Shuangcheng Liang,Sidi Wang,Ruoting Zhan,Kui Wang
出处
期刊:International Journal of Biological Macromolecules [Elsevier BV]
卷期号:224: 653-666 被引量:3
标识
DOI:10.1016/j.ijbiomac.2022.10.154
摘要

Two novel Bacillus glycoside hydrolase family 53 (GH53) endo-1,4-β-galactanases (Bs936 and Bs4828) were identified. The recombinant Bs936 and Bs4828 displayed maximal activities at pH 5.5/55 °C and pH 6.5/65 °C, respectively. Stability analyses revealed that the enzymes were stable between pH 4.5–10, retaining over 80% activities after 12 h incubation at 25 °C. Moreover, Bs936 maintained about 75% activity after being treated at 45 °C for 2 h, while Bs4828 kept full activity after 4 h of incubation at 50 °C. Importantly, Bs936 and Bs4828 exhibited good activity towards β-1,4-galactan, showing specific activities of 1859.46- and 3110.79 U/mg towards potato galactan, respectively. The corresponding Km and kcat/Km were 4.93 mg/mL and 296.35 mL/mg/s for Bs936, and 6.60 mg/mL and 707.12 mL/mg/s for Bs4828. Extensive synergy assays revealed that each endo-1,4-β-galactanase acted synergistically with the other β-galactosidase (Bs937 or Bs4829), endo-1,5-α-L-arabinosidase (BsAbn2) and an endoxylanase/endoglucanase+cellulase mixture (BaXynA + LsCel) towards the hydrolysis of galactan, debranched arabinan/arabinan, and pretreated corn stover, respectively. The corresponding degrees of synergy were 1.28, 1.17/1.09, and 1.15 for Bs486 and 1.55, 1.04/1.08, and 1.11 for Bs4828, respectively. This study expands the repertoire of GH53 enzymes, and demonstrates the role of two highly active endo-1,4-β-galactanases in polysaccharides hydrolysis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
nanfeng完成签到,获得积分10
1秒前
1秒前
waayu完成签到 ,获得积分10
2秒前
迷人芙蓉发布了新的文献求助10
3秒前
量子星尘发布了新的文献求助10
3秒前
搜集达人应助kassidy采纳,获得10
4秒前
4秒前
罗那完成签到,获得积分10
4秒前
5秒前
vv关闭了vv文献求助
6秒前
科研通AI2S应助研友_RLNXOZ采纳,获得10
6秒前
852应助Whale采纳,获得10
6秒前
7秒前
王者归来发布了新的文献求助30
7秒前
8秒前
猪猪hero应助鲤鱼安青采纳,获得10
8秒前
失眠水风完成签到,获得积分10
8秒前
8秒前
Rondab应助shinn采纳,获得10
9秒前
westbobo发布了新的文献求助10
9秒前
10秒前
11秒前
12秒前
啊啊啊发布了新的文献求助10
14秒前
科目三应助westbobo采纳,获得10
14秒前
14秒前
15秒前
15秒前
15秒前
15秒前
16秒前
Ion发布了新的文献求助10
16秒前
16秒前
16秒前
bei发布了新的文献求助10
18秒前
共享精神应助李治稳采纳,获得10
19秒前
zhuzhuxia发布了新的文献求助10
19秒前
研友_RLNXOZ发布了新的文献求助10
19秒前
19秒前
20秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975339
求助须知:如何正确求助?哪些是违规求助? 3519670
关于积分的说明 11199199
捐赠科研通 3256002
什么是DOI,文献DOI怎么找? 1798043
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305