Asthenopia is a group of eye discomfort syndromes with complex clinical manifestations, accompanied with systemic symptoms or not. It is caused by diverse influencing factors and occurs upon overuse of the eyes. Over the past decade, significant changes have appeared in the etiology, clinical manifestation and treatment of asthenopia with great changes in the society and lifestyles. The Chinese Optometric Association of Chinese Ophthalmological Society and the Optometry Group of Chinese Ophthalmologist Association have organized nationwide experts in the field to thoroughly discuss the latest researches on asthenopia. Consensus opinions have been formed on the causes, influencing factors, clinical manifestations, diagnosis and treatment of asthenopia, following the definition of asthenopia in the International Classification of Diseases, so as to provide guidance for the clinical diagnosis and treatment of asthenopia.