As stirling coolers are widely used in military, aerospace and other application, the demand for the driving circuits is rapidly increasing. And it has become more urgent to improve the performance of driving circuits, including driving capability, noise control and temperature control accuracy. Therefore, this paper presents the stirling cooler driving circuit based on microcontroller unit (MCU), which adopts the fuzzy proportional-integral-derivative (PID) control algorithm to realize the closed-loop control of the temperature. Firstly, the proposed driving circuit scheme and core devices' selection are introduced in detail. Secondly, the principle and characteristics of the control scheme based on fuzzy PID control algorithm are analyzed and established. Finally, a detailed simulation study in MATLAB/Simulink is performed to investigate the performance of the fuzzy PID controller. The results illustrate that the performance of proposed control approach in comparison to the traditional PID has been greatly improved, with smaller overshoot, lower settling time and better dynamic performance.