Cry1Ac公司
苏云金杆菌
生物
棉铃虫
苏力菌棉花
有害生物分析
转基因作物
拉伤
基因
基因表达
微生物学
遗传学
转基因
生殖器鳞翅目
植物
细菌
解剖
作者
Jiao Tang,Jie Lu,Caihong Zhang,Dandan Zhang,Siqi Yu,Fei Fang,Zaw Lin Naing,Ei Thinzar Soe,Zijian Ding,Gemei Liang
标识
DOI:10.1016/j.ijbiomac.2023.127668
摘要
Rapid evolution of pest resistance to Bt insecticidal proteins presents a serious threat to the sustainable use of Bt crops. The cotton bollworm has been extensively exposed to Bt cotton worldwide and has evolved resistance in laboratory and field. Previous studies have highlighted the significant roles played by the ABC transporter proteins in Bt resistance. In this study, the ORF of HaABCB1 was cloned and analyzed. The expression of HaABCB1 was detected in all developmental stages and tissues, with the highest expression in third instar larvae stage and hindgut tissue. Compared with susceptible strain, a remarkable decrease of HaABCB1 expression in Cry1Ac resistant strain while no significant change in Cry2Ab resistant strain were found. The HaABCB1 expression reduced after susceptible larvae induced by Cry1Ac, but no obvious expression changes after Cry2Ab exposure. RNAi-mediated down-regulation of HaABCB1 could lead to a significant reduction in larval susceptibility to Cry1Ac, but not to Cry2Ab, in susceptible strain. Genetic linkage analysis confirmed that decreased expression of the HaABCB1 mediates resistance to Cry1Ac, but not Cry2Ab resistance. This knowledge contributes to better understanding of the complex molecular mechanisms underlying Bt resistance and provide theoretical foundation for the development of new strategies for pest resistance management.
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