In-vitro study for Ibuprofen encapsulation, controlled release and cytotoxicity improvement using excipient-drugs mixed micelle

The dye-drug combinations are widely used in pharmaceutics, but as a mixed micelle, little attention has been dedicated to improving poorly water-soluble pharmaceuticals and cytotoxicity in the pharmaceutical and biotechnology industries. This study examined the influence of amphiphilic dyes and medicines in aqueous media. Anionic tartrazine (TAZ) was combined with cationic diphenhydramine hydrochloride (DPC) and cetirizine hydrochloride (CTZ) to form mixed micelles. As revealed by the mixed micelles of TAZ-DPC and TAZ-CTZ, TAZ and DPC/CTZ exhibited a more pronounced synergistic relationship. Mixed micelles increased the binding constant (LogKb) of the anti-inflammatory Ibuprofen (IBF) drug by raising the αTAZ 0.0–1.0 in the aqueous environment. At a very high mole fraction of αTAZ 0.99, the highest LogKb values attained were 4.3 and 4.1. The present study used molecular docking to portray IBF's interaction with TAZ, DPC, CTZ, and TAZ-DPC and TAZ-CTZ combinations. Electrostatic contact made IBF more bound in TAZ and TAZ-DPC mixtures. TAZ-DPC had better encapsulation efficiency (%EE) than TAZ-CTZ at αTAZ 0.9, indicating an electrostatic interaction between IBF and TAZ rich mixed micelles. After 50 h, the controlled release of IBF in PBS at pH 7.4 was about 46% from both mixed micelles. Furthermore, with αTAZ 0.9 at 25 mmolL−1, cell viability in TAZ-DPC and TAZ-CTZ mixed micelles was increased by 78% and 72%, respectively, in the presence of IBF. Increasing TAZ concentrations may have reduced IBF cytotoxicity as seen by the enhanced cell viability. The current study also showed that cytotoxicity could be decreased and the number of excipients in a drug delivery system could be decreased by combining pharmaceuticals with amphiphilic dye.