化学
树突状细胞
免疫系统
信使核糖核酸
细胞生物学
皮克林乳液
细胞内
生物化学
乳状液
免疫学
生物
基因
作者
Sihua Wu,Yan Zhou,Naoki Asakawa,Mei Wen,Yang Yang,Yali Ming,Tiantian Song,Wansong Chen,Guanghui Ma,Yufei Xia
标识
DOI:10.1016/j.jconrel.2024.07.051
摘要
mRNA delivery systems, such as lipid nanoparticle (LNP), have made remarkable strides in improving mRNA expression, whereas immune system activation operates on a threshold. Maintaining a delicate balance between antigen expression and dendritic cell (DC) activation is vital for effective immune recognition. Here, a water-in-oil-in-water (w/o/w) Pickering emulsion stabilized with calcium phosphate nanoparticles (CaP-PME) is developed for mRNA delivery in cancer vaccination. CaP-PME efficiently transports mRNA into the cytoplasm, induces pro-inflammatory responses and activates DCs by disrupting intracellular calcium/potassium ions balance. Unlike LNP, CaP-PME demonstrates a preference for DCs, enhancing their activation and migration to lymph nodes. It elicits interferon-γ-mediated CD8
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