已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

The role of EndophilinA1 in chronic unpredicted mild stress-induced depression model mice

尾部悬挂试验 行为绝望测验 海马体 开阔地 尼氏体 神经炎症 内分泌学 内科学 生物 化学 医学 病理 炎症 染色 抗抑郁药
作者
Mengqing Liu,Yi Ling,Yue Zhang,Lulu Liu,Yue Qiu,Yi Liu,Yanyan Yin
出处
期刊:International Immunopharmacology [Elsevier]
卷期号:124: 111023-111023 被引量:1
标识
DOI:10.1016/j.intimp.2023.111023
摘要

Depression is a common mental disease, accompanied by anxiety and persistent depression. Endophilin A1 (EPA1) is a brain-specific protein enriched in synaptic terminals that is primarily expressed in the central nervous system. It has been reported that EPA1 is involved in neurotransmitter release, which indicates that the protein may be involved in depression. However, it is unclear whether EPA1 is implicated in the development of depression.The mice depression model was established by chronic unpredicted mild stress (CUMS). Depression-like behaviors were detected by sucrose preference test (SPT), forced swim test (FST), tail-suspension test (TST) and open-field test (OFT). Neuronal histopathology was applied by hematoxylin and eosin stain (H&E), and Nissl stain. EPA1, NLRP1 inflammatory complexes, NADPH oxidase2 (NOX2), synaptic-related protein expression of the mice were tested by western blot. Immunofluorescence was applied to detect the expression of EPA1 and ROS in mice hippocampus. EPA1 knockdown was performed by an adeno-associated virus (AAV) vector containing EPA1-shRNA-EGFP infusion.CUMS exposure induced depressive-like behaviors and increased the expression of EPA1 in the hippocampus. Knockdown hippocampal EPA1 ameliorated CUMS-induced depressive-like behaviors, decreased calcium (Ca2+) overload, decreased ROS generation and NOX2 expression, inhibited NLRP1 inflammasome-driven neuroinflammation, and restored the levels of BDNF, PSD95, GAP-43, SYN, and MAP-2 in the hippocampus.EPA1 contributes to CUMS induced depressive-like behaviors and the mechanism may be related to NLRP1 inflammasome-driven inflammatory response, regulating calcium ion homeostasis and ROS generation, and alleviating synaptic function damage. This indicated that EPA1 may participate in the occurrence and development of depression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
楚天阔发布了新的文献求助30
刚刚
拾新发布了新的文献求助10
1秒前
英姑应助卫念烟采纳,获得30
3秒前
3秒前
养乐多敬你完成签到 ,获得积分10
4秒前
蔚欢完成签到 ,获得积分10
4秒前
cjx完成签到,获得积分10
4秒前
啊奶糖完成签到,获得积分10
6秒前
6秒前
Hehhhh完成签到,获得积分10
7秒前
高贵书南发布了新的文献求助10
7秒前
大个应助科研通管家采纳,获得10
8秒前
楚天阔完成签到,获得积分10
10秒前
自信飞柏完成签到 ,获得积分10
11秒前
忧虑的羊发布了新的文献求助10
11秒前
hsiao_yang完成签到,获得积分10
12秒前
别找了睡觉吧完成签到 ,获得积分10
13秒前
Litm完成签到 ,获得积分10
14秒前
marco完成签到 ,获得积分10
14秒前
15秒前
fanfan完成签到,获得积分10
15秒前
科研通AI2S应助楚天阔采纳,获得10
17秒前
JoeyCory发布了新的文献求助10
20秒前
22秒前
Vito完成签到,获得积分10
23秒前
26秒前
27秒前
Driscoll完成签到 ,获得积分10
29秒前
29秒前
lxz3131发布了新的文献求助10
30秒前
彩色莞完成签到 ,获得积分10
34秒前
hsiao_yang发布了新的文献求助10
36秒前
Ploaris完成签到 ,获得积分10
36秒前
JoeyCory完成签到,获得积分10
39秒前
学不完了完成签到 ,获得积分10
40秒前
啦啦啦完成签到,获得积分10
42秒前
喝奶茶睡不着完成签到,获得积分10
42秒前
44秒前
科研通AI2S应助英勇的汉堡采纳,获得50
45秒前
科研通AI2S应助lxz3131采纳,获得10
45秒前
高分求助中
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Handbook of Qualitative Cross-Cultural Research Methods 600
Chen Hansheng: China’s Last Romantic Revolutionary 500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3139398
求助须知:如何正确求助?哪些是违规求助? 2790314
关于积分的说明 7794847
捐赠科研通 2446748
什么是DOI,文献DOI怎么找? 1301366
科研通“疑难数据库(出版商)”最低求助积分说明 626153
版权声明 601141