亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Mitochondria immobilized carbonized polymer dots for ratio-metric tracking mitophagy

粒体自噬 荧光 生物物理学 线粒体 聚合物 化学 材料科学 纳米技术 生物 有机化学 生物化学 量子力学 物理 细胞凋亡 自噬
作者
Xueting Luo,Wentao Xiang,Yi Lu,Erjing Wang,Jun You,Cao Li,Ziqiang Xu
出处
期刊:Chemical Engineering Journal [Elsevier BV]
卷期号:488: 151169-151169 被引量:5
标识
DOI:10.1016/j.cej.2024.151169
摘要

Mitophagy is essential for adjusting the homeostasis of mitochondria and real-time monitoring mitophagy process is essential for investigating mitochondrial-related physiology/pathology status. However, most fluorescent probes are focused on organic dyes, while toxicity, water-solubility, and photobleaching issues are required to be addressed. Herein, fluorescent carbonized polymer dots (CPDs) that can visualize the process of mitophagy with ratio-metric mode and are independent of the mitochondria membrane potential have been fabricated for the first time. The CPDs are composed of a crosslinked polymer core and phenanthroline-like structure, which can be firmly immobilized on mitochondria, due to their lipophilic polymer core. Obvious pH-sensitive and ratio-metric fluorescent features within pH 5 and 8 are observed in the CPDs. The pKa of CPDs is determined to be 6.67, which is highly consistent with neutral physiological pH, which can satisfy the requirement of monitoring mitophagy. Subsequently, we have found that the CPDs can be targeted to mitochondria and precisely monitor the pH perturbation during the mitophagy induced by CCCP or rapamycin. Interestingly, the process of CCCP elicited mitophagy relieved by the methyladenine or chloroquine can be also monitored by the CPDs. The acidification of mitochondria at this condition, combined with the rationally designed CPDs may provide a promising direction for investigating the mechanism of mitochondria in some bioprocesses.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
12秒前
lijiawei发布了新的文献求助10
14秒前
Criminology34发布了新的文献求助50
16秒前
睡觉补充能量完成签到,获得积分10
16秒前
fengfenghao完成签到,获得积分10
22秒前
小凯完成签到 ,获得积分10
25秒前
jueshadi完成签到 ,获得积分10
25秒前
朵朵完成签到,获得积分10
27秒前
32秒前
36秒前
dong发布了新的文献求助10
37秒前
39秒前
39秒前
39秒前
aaaaal发布了新的文献求助10
41秒前
43秒前
annaanna完成签到 ,获得积分10
45秒前
Criminology34发布了新的文献求助50
47秒前
ccc完成签到 ,获得积分10
47秒前
49秒前
YY发布了新的文献求助10
49秒前
Gin发布了新的文献求助10
52秒前
aaaaal完成签到,获得积分10
52秒前
Becky完成签到 ,获得积分10
55秒前
56秒前
杨北风完成签到 ,获得积分20
56秒前
科研通AI6应助科研通管家采纳,获得10
57秒前
58秒前
共享精神应助科研通管家采纳,获得10
58秒前
58秒前
所所应助科研通管家采纳,获得10
58秒前
58秒前
58秒前
Hello应助科研通管家采纳,获得10
58秒前
小蘑菇应助科研通管家采纳,获得10
58秒前
58秒前
ye发布了新的文献求助20
1分钟前
1分钟前
1分钟前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Architectural Corrosion and Critical Infrastructure 1000
Early Devonian echinoderms from Victoria (Rhombifera, Blastoidea and Ophiocistioidea) 1000
By R. Scott Kretchmar - Practical Philosophy of Sport and Physical Activity - 2nd (second) Edition: 2nd (second) Edition 666
Electrochemistry: Volume 17 600
Physical Chemistry: How Chemistry Works 500
SOLUTIONS Adhesive restoration techniques restorative and integrated surgical procedures 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 4944655
求助须知:如何正确求助?哪些是违规求助? 4209521
关于积分的说明 13085355
捐赠科研通 3989302
什么是DOI,文献DOI怎么找? 2184055
邀请新用户注册赠送积分活动 1199418
关于科研通互助平台的介绍 1112457