油菜素甾醇
磷酸化
细胞生物学
生长素
信号转导
长时程增强
生物
蛋白质水解
生物化学
化学
拟南芥
突变体
酶
基因
受体
作者
Ming Ma,Shao-Yan Shen,Chen Bai,Wei-Qing Wang,Xiao-Hui Feng,Jie-Zheng Ying,Xian-Jun Song
出处
期刊:Cell Reports
[Elsevier]
日期:2023-03-01
卷期号:42 (3): 112187-112187
标识
DOI:10.1016/j.celrep.2023.112187
摘要
Grain size is a key component of grain yield and quality in crops. Several core players of auxin signaling have been revealed to modulate grain size; however, to date, few genetically defined pathways have been reported, and whether phosphorylation could boost degradation of Aux/IAA proteins is uncertain. Here, we show that TGW3 (also called OsGSK5) interacts with and phosphorylates OsIAA10. Phosphorylation of OsIAA10 facilitates its interaction with OsTIR1 and subsequent destabilization, but this modification hinders its interaction with OsARF4. Our genetic and molecular evidence identifies an OsTIR1-OsIAA10-OsARF4 axis as key for grain size control. In addition, physiological and molecular studies suggest that TGW3 mediates the brassinosteroid response, the effect of which can be relayed through the regulatory axis. Collectively, these findings define a auxin signaling pathway to regulate grain size, in which phosphorylation of OsIAA10 enhances its proteolysis and potentiates OsIAA10-OsARF4-mediated auxin signaling.
科研通智能强力驱动
Strongly Powered by AbleSci AI