适体
限制
纳米技术
计算机科学
检出限
功能(生物学)
生物系统
计算生物学
材料科学
化学
生物
工程类
遗传学
机械工程
色谱法
作者
Weiqi Zhang,Yi‐Dan Tu,Hong Liu,Rui Liu,Xiaojin Zhang,Lei Jiang,Yu Huang,Fan Xia
标识
DOI:10.1002/anie.202316434
摘要
Aptamer-based probes are pivotal components in various sensing strategies, owing to their exceptional specificity and versatile programmable structure. Nevertheless, numerous aptamer-based probes usually offer only a single function, limiting their capacity to meet the diverse requirements of multi-faceted sensing systems. Here, we introduced supersandwich DNA probes (SSW-DNA), designed and modified on the outer surface of nanochannels with hydrophobic inner walls, enabling dual functionality: qualitative detection for on-site analysis and quantitative detection for precise analysis. The fragmented DNAs resulting from the target recognition, are subsequently identified through lateral flow assays, enabling robust on-site qualitative detection of microcystin-LR with an impressively low limit of detection (LOD) at 0.01 μg/L. Meanwhile, the nanochannels enable highly sensitive quantification of microcystin-LR through the current analysis, achieving an exceptionally low LOD at 2.5×10
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