链霉素
庆大霉素
青霉素
抗生素
超氧化物
间充质干细胞
氨基糖苷
微生物学
化学
生物
细胞生物学
生物化学
酶
作者
Inna Zumberg,Larisa Baiazitova,Vratislav Čmiel
出处
期刊:IFMBE proceedings
日期:2024-01-01
卷期号:: 29-36
标识
DOI:10.1007/978-3-031-49068-2_4
摘要
Antibiotics have become an integral part of routine cell culturing. Penicillin-streptomycin mixture and gentamicin are widely used to prevent bacterial contamination of cells. A lot of publications have elucidated the effect of these antibiotics in culture medium on differentiation and metabolism of mesenchymal stem cells (MSCs). However, much less is known about their influence on cell migration and mitochondrial superoxide production. In this study, MSCs from human adipose tissue were cultured in media containing mostly used concentrations of mentioned antibiotics. In total, two concentrations of penicillin-streptomycin (100 U·ml−1–100 µg·ml−1, 200 U·ml−1–200 µg·ml−1) and gentamicin (10 µg·ml−1, 50 µg·ml−1) were tested. The acquisition of microscopic images was performed using Leica TCS SP8 X confocal laser scanning microscope. The MitoSOX Red indicator was used to observe mitochondrial superoxide. Image processing and statistical analysis was performed using MATLAB software. We found that the addition of these antibiotics to the medium negatively affects the cell migration velocity in a dose-dependent manner, with penicillin-streptomycin inhibiting cell migration less than gentamicin. Also, penicillin-streptomycin and gentamicin induce mitochondrial superoxide production in cells.
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