化学
酶
异源表达
异源的
生物合成
小檗碱
笼子
生物化学
立体化学
氧化酶试验
基因
重组DNA
数学
组合数学
作者
Shiyuan Wang,Min Wang,Chengbao Duan,Yongpeng Yao,Jinwei Ren,Ling Liu,Yuanyuan Pan,Gang Liu
出处
期刊:Organic Letters
[American Chemical Society]
日期:2024-01-12
卷期号:26 (3): 642-646
被引量:4
标识
DOI:10.1021/acs.orglett.3c03966
摘要
Oxosorbicillinol and cage-like acresorbicillinol C are bioactive sorbicillinoids produced by Acremonium chrysogenum. We found that a berberine bridge enzyme-like oxidase AcsorD was responsible for their biosynthesis by gene deletion and heterologous expression. AcsorD catalyzed oxidation of sorbicillinol to form oxosorbicillinol in in vitro assays, which was successively condensed with sorbicillinol to form acresorbicillinol C spontaneously. Finally, site-directed mutation revealed that Tyr525 was the key residue in the catalysis of the oxidation reaction and unlocking cage-like acresorbicillinol C production.
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