Marinimicrococcus flavescens gen. nov., sp. nov., a new member of the family Geminicoccaceae, isolated from a marine sediment of the South China Sea

A Gram-stain-negative, catalase-positive and oxidase-positive, nonmotile, aerobic, light yellow, spherical-shaped bacterial strain with no flagella, designated strain YIM 152171 T , was isolated from sediment of the South China Sea. Colonies were smooth and convex, light yellow and circular, and 1.0–1.5×1.0–1.5 µm in cell diameter after 7 days of incubation at 28°C on YIM38 media supplemented with sea salt. Colonies could grow at 20–45°C (optimum 28–35°C) and pH 6.0–11.0 (optimum, pH 7.0–9.0), and they could proliferate in the salinity range of 0–6.0 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C 18 : 1 ω 7 c /C 18 : 1 ω6 c ), C 18 : 1 ω7 c 11-methyl, C 16 : 0 , C 16 : 1 ω11 c , C 16 : 1 ω5 c , C 17 : 1 ω6 c and C 18 : 1 ω5 c . The respiratory quinone was ubiquinone 10, and the polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, one unidentified phospholipid and one unidentified aminolipid. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain YIM 152171 T within the order Rhodospirillales in a distinct lineage that also included the genus Geminicoccus . The 16S rRNA gene sequence similarities of YIM 152171 T to those of Arboricoccus pini , Geminicoccus roseus and Constrictibacter antarcticus were 92.17, 89.25 and 88.91 %, respectively. The assembled draft genome of strain YIM 152171 T had 136 contigs with an N50 value of 134704 nt, a total length of 3 001 346 bp and a G+C content of 70.27 mol%. The phylogenetic, phenotypic and chemotaxonomic data showed that strain YIM 152171 T (=MCCC 1K08488 T =KCTC 92884 T ) represents a type of novel species and genus for which we propose the name Marinimicrococcus gen. nov., sp. nov.