Multi-channel nanopore sensing platform for single-molecule protein fingerprinting

Fingerprinting proteins at a single-molecule level holds the potential to unveil profound details into key biological features that have remained elusive using conventional proteomic methods such as mass spectrometry. Enzyme-free translocation of denatured proteins through nanopores can be an alternative to enzyme-driven protein transport because it can provide a faster and higher throughput survey of the proteome by fingerprinting the signatures of each of its protein constituents, without the need for sequencing every amino acid.