离体
FGF1型
体内
血管内皮生长因子
伤口愈合
成纤维细胞生长因子
新生血管
生长因子
血管内皮生长因子A
血管生成
微血管
碱性成纤维细胞生长因子
血管内皮生长因子受体
癌症研究
生物
医学
外科
成纤维细胞生长因子受体
内科学
受体
生物技术
作者
Sandra Tejedor,Maria Wågberg,Catarina Correia,Karin Åvall,Mikko Hölttä,Leif Hultin,M. Lerche,Nigel Davies,Nils Bergenhem,Arjan Snijder,Tom Marlow,Pierre Dönnes,Regina Fritsche‐Danielson,Jane Synnergren,Karin Jennbacken,Kenny M. Hansson
出处
期刊:Cells
[MDPI AG]
日期:2024-02-27
卷期号:13 (5): 414-414
标识
DOI:10.3390/cells13050414
摘要
Background: Diabetic foot ulcers (DFU) pose a significant health risk in diabetic patients, with insufficient revascularization during wound healing being the primary cause. This study aimed to assess microvessel sprouting and wound healing capabilities using vascular endothelial growth factor (VEGF-A) and a modified fibroblast growth factor (FGF1). Methods: An ex vivo aortic ring rodent model and an in vivo wound healing model in diabetic mice were employed to evaluate the microvessel sprouting and wound healing capabilities of VEGF-A and a modified FGF1 both as monotherapies and in combination. Results: The combination of VEGF-A and FGF1 demonstrated increased vascular sprouting in the ex vivo mouse aortic ring model, and topical administration of a combination of VEGF-A and FGF1 mRNAs formulated in lipid nanoparticles (LNPs) in mouse skin wounds promoted faster wound closure and increased neovascularization seven days post-surgical wound creation. RNA-sequencing analysis of skin samples at day three post-wound creation revealed a strong transcriptional response of the wound healing process, with the combined treatment showing significant enrichment of genes linked to skin growth. Conclusion: f-LNPs encapsulating VEGF-A and FGF1 mRNAs present a promising approach to improving the scarring process in DFU.
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