金黄色葡萄球菌
铜绿假单胞菌
微生物学
抗菌剂
生物膜
伤口愈合
益生菌
白色念珠菌
医学
生物
细菌
免疫学
遗传学
作者
Siyuan Tao,Sixuan Zhang,Kongchang Wei,Katharina Maniura‐Weber,Zhihao Li,Qun Ren
标识
DOI:10.1002/adhm.202400921
摘要
Abstract Wound infections pose a significant challenge in healthcare, and traditional antibiotic treatments often result in the development of resistant pathogens. Addressing this gap, ProGel is introduced, a living hydrogel created by entrapping probiotic Lactobacillus plantarum as a therapeutic component within a gelatin matrix. With a double‐syringe system, ProGel can be easily mixed and applied, conforming swiftly to any wound shape and forming hydrogel in situ. It also demonstrates robust mechanical and self‐healing properties owing to the Schiff‐base bonds. ProGel sustains more than 80% viability of the entrapped L. plantarum while restricting their escape from the hydrogel. After a week of storage, more than 70% viability of the entrapped L. plantarum is preserved. Importantly, ProGel exhibits broad‐spectrum antimicrobial efficacy against pathogens commonly associated with wound infections, i.e., Pseudomonas aeruginosa (7Log reduction), Staphylococcus aureus (3‐7Log reduction), and Candida albicans (40–70% reduction). Moreover, its cytocompatibility is affirmed through coculture with human dermal fibroblasts. The effectiveness of ProGel is further highlighted in more clinically relevant tests on human skin wound models infected with P. aeruginosa and S. aureus , where it successfully prevents the biofilm formation of these pathogens. This study showcases an injectable living hydrogel system for the management of complex wound infections.
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