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Longitudinal analysis of exposure to a low concentration of oxytetracycline on the zebrafish gut microbiome

土霉素 斑马鱼 微生物群 生物 四环素 微生物学 抗生素 基因组 细菌 基因 遗传学
作者
Masood ur Rehman Kayani,Yu Kan,Yushu Qiu,Xiaogang Yu,Lei Chen,Lisu Huang
出处
期刊:Frontiers in Microbiology [Frontiers Media]
卷期号:13 被引量:4
标识
DOI:10.3389/fmicb.2022.985065
摘要

Oxytetracycline, a widely produced and administered antibiotic, is uncontrollably released in low concentrations in various types of environments. However, the impact of exposure to such low concentrations of antibiotics on the host remains poorly understood. In this study, we exposed zebrafish to a low concentration (5,000 ng/L) of oxytetracycline for 1 month, collected samples longitudinally (Baseline, and Days 3, 6, 9, 12, 24, and 30), and elucidated the impact of exposure on microbial composition, antibiotic resistance genes, mobile genetic elements, and phospholipid metabolism pathway through comparison of the sequenced data with respective sequence databases. We identified Pseudomonas aeruginosa , a well-known pathogen, to be significantly positively associated with the duration of oxytetracycline exposure (Adjusted P = 5.829e −03 ). Several tetracycline resistance genes (e.g., tetE ) not only showed significantly higher abundance in the exposed samples but were also positively associated with the duration of exposure (Adjusted P = 1.114e −02 ). Furthermore, in the exposed group, the relative abundance of genes involved in phospholipid metabolism had also decreased. Lastly, we characterized the impact of exposure on zebrafish intestinal structure and found that the goblet cell counts were decreased (~82%) after exposure. Overall, our results show that a low concentration of oxytetracycline can increase the abundance of pathogenic bacteria and lower the abundance of key metabolic pathways in the zebrafish gut microbiome that can render them prone to bacterial infections and health-associated complications.

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