费斯特共振能量转移
渡线
DNA
单分子微动
分子
化学
材料科学
计算机科学
物理
荧光
有机化学
生物化学
光学
人工智能
作者
Xueqiao Li,Libang Wang,Wenna Wu,Huajie Liu,Chunhua Xu,Tao Zhang
出处
期刊:Soft Matter
[The Royal Society of Chemistry]
日期:2024-01-01
摘要
In this study, we utilized the origami technique to integrate various types of spacers into the double-stranded crossover and examined their flexibilities using single-molecule fluorescence resonance energy transfer (smFRET). We discovered that for the traditional Holliday Junction connection with zero-base spacers, the inter-structural angle measures 58.7 degrees, which aligns well with previous crystallographic research. When introducing non-complementary double-stranded spacers as a free leash, we observed that longer spacers resulted in a more relaxed connection. In contrast, when using complementary segments, the two origami structures rotated as the number of base pairs increased, reflecting the structural characteristics of the B-duplex. Our findings indicate that a stable intramolecular duplex requires a minimum of 5 base pairs. Overall, our results highlight the potential for re-engineering crossovers and designing materials that can change volume with shrink-swell capabilities, as well as applications in torque sensing using short DNA duplexes.
科研通智能强力驱动
Strongly Powered by AbleSci AI